BMRB Entry 17510

Name: 1H, 13C and 15N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72
Published: 2011-07-07

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17510

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C and 15N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72

Deposition date:

2011-03-04

Original release date:

2011-07-07

Authors:

Werner-Allen, Jon; Zhou, Pei

Citation:

Citation: Werner-Allen, Jon; Zhou, Pei. "(1)H, (13)C and (15)N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72." Biomol. NMR Assignments 6, 57-61 (2012).
PubMed: 21732054

Assembly members:

Assembly members:
Ssu72, polymer, 198 residues, Formula weight is not available
PHOSPHATE ION, non-polymer, 94.971 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ssu72: GSHMTDPSKLAVAVVCSSNM NRSMEAHNFLAKKGFNVRSY GTGERVKLPGMAFDKPNVYE FGTKYEDIYRDLESKDKEFY TQNGLLHMLDRNRRIKKCPE RFQDTKEQFDIIVTVEERVY DLVVMHMESMESVDNRPVHV LNVDVVDNAEDALMGAFVIT DMINMMAKSTDLDNDIDELI QEFEERRKRVILHSVLFY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	791
15N chemical shifts	177
1H chemical shifts	1287

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Drosophila melanogaster Ssu72	1
2	inorganic phosphate	2

Entities:

Entity 1, Drosophila melanogaster Ssu72 198 residues - Formula weight is not available

1

GLY

SER

HIS

MET

THR

ASP

PRO

SER

LYS

LEU

2

ALA

VAL

ALA

VAL

CYS

SER

ASN

MET

3

ASN

ARG

SER

MET

GLU

ALA

HIS

ASN

PHE

LEU

4

ALA

LYS

GLY

PHE

ASN

VAL

ARG

SER

TYR

5

GLY

THR

GLY

GLU

ARG

VAL

LYS

LEU

PRO

GLY

6

MET

ALA

PHE

ASP

LYS

PRO

ASN

VAL

TYR

GLU

7

PHE

GLY

THR

LYS

TYR

GLU

ASP

ILE

TYR

ARG

8

ASP

LEU

GLU

SER

LYS

ASP

LYS

GLU

PHE

TYR

9

THR

GLN

ASN

GLY

LEU

HIS

MET

LEU

ASP

10

ARG

ASN

ARG

ILE

LYS

CYS

PRO

GLU

11

ARG

PHE

GLN

ASP

THR

LYS

GLU

GLN

PHE

ASP

12

ILE

VAL

THR

VAL

GLU

ARG

VAL

TYR

13

ASP

LEU

VAL

MET

HIS

MET

GLU

SER

MET

14

GLU

SER

VAL

ASP

ASN

ARG

PRO

VAL

HIS

VAL

15

LEU

ASN

VAL

ASP

VAL

ASP

ASN

ALA

GLU

16

ASP

ALA

LEU

MET

GLY

ALA

PHE

VAL

ILE

THR

17

ASP

MET

ILE

ASN

MET

ALA

LYS

SER

THR

18

ASP

LEU

ASP

ASN

ASP

ILE

ASP

GLU

LEU

ILE

19

GLN

GLU

PHE

GLU

ARG

LYS

ARG

VAL

20

ILE

LEU

HIS

SER

VAL

LEU

PHE

TYR

Entity 2, inorganic phosphate - O4 P - 94.971 Da.

1

PO4

Samples:

sample_2: Ssu72, [U-100% 13C; U-100% 15N], 1 mM; H2O 95%; D2O 5%; Sodium phosphate 25 mM; Potassium chloride 25 mM; dithiothreitol 2 mM

sample_3: Ssu72, [U-100% 13C; U-100% 15N], 1 mM; D2O 100%; Sodium phosphate 25 mM; Potassium chloride 25 mM; dithiothreitol 2 mM

sample_4: Ssu72, [U-10% 13C], 1 mM; H2O 95%; D2O 5%; Sodium phosphate 25 mM; Potassium chloride 25 mM; dithiothreitol 2 mM

sample_1: Ssu72, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mM; H2O 95%; D2O 5%; Sodium phosphate 25 mM; Potassium chloride 25 mM; dithiothreitol 2 mM

sample_conditions_1: ionic strength: 75 mM; pH: 8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HN(CA)HA	sample_2	isotropic	sample_conditions_1
3D HN(COCA)HA	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks