BMRB Entry 17499
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17499
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Citation: Habenstein, Birgit; Wasmer, Christian; Bousset, Luc; Sourigues, Yannick; Schutz, Anne; Loquet, Antoine; Meier, Beat; Melki, Ronald; Bockmann, Anja. "Extensive de novo solid-state NMR assignments of the 33 kDa C-terminal domain of the Ure2 prion." J. Biomol. NMR 51, 235-243 (2011).
PubMed: 21805376
Assembly members:
Ure2p70-354, polymer, 295 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 3A
Entity Sequences (FASTA):
Ure2p70-354: MNNDNENNIKNTLEQHRQQQ
QAFSDMSHVEYSRITKFFQE
QPLEGYTLFSHRSAPNGFKV
AIVLSELGFHYNTIFLDFNL
GEHRAPEFVSVNPNARVPAL
IDHGMDNLSIWESGAILLHL
VNKYYKETGNPLLWSDDLAD
QSQINAWLFFQTSGHAPMIG
QALHFRYFHSQKIASAVERY
TDEVRRVYGVVEMALAERRE
ALVMELDTENAAAYSAGTTP
MSQSRFFDYPVWLVGDKLTI
ADLAFVPWNNVVDRIGINIK
IEFPEVYKWTKHMMRRPAVI
KALRGERGSHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 751 |
| 15N chemical shifts | 192 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ure2p70-354 | 1 |
Entities:
Entity 1, Ure2p70-354 295 residues - Formula weight is not available
RGSHHHHHH at the C-terminus is the non-native His-tag
| 1 | MET | ASN | ASN | ASP | ASN | GLU | ASN | ASN | ILE | LYS | ||||
| 2 | ASN | THR | LEU | GLU | GLN | HIS | ARG | GLN | GLN | GLN | ||||
| 3 | GLN | ALA | PHE | SER | ASP | MET | SER | HIS | VAL | GLU | ||||
| 4 | TYR | SER | ARG | ILE | THR | LYS | PHE | PHE | GLN | GLU | ||||
| 5 | GLN | PRO | LEU | GLU | GLY | TYR | THR | LEU | PHE | SER | ||||
| 6 | HIS | ARG | SER | ALA | PRO | ASN | GLY | PHE | LYS | VAL | ||||
| 7 | ALA | ILE | VAL | LEU | SER | GLU | LEU | GLY | PHE | HIS | ||||
| 8 | TYR | ASN | THR | ILE | PHE | LEU | ASP | PHE | ASN | LEU | ||||
| 9 | GLY | GLU | HIS | ARG | ALA | PRO | GLU | PHE | VAL | SER | ||||
| 10 | VAL | ASN | PRO | ASN | ALA | ARG | VAL | PRO | ALA | LEU | ||||
| 11 | ILE | ASP | HIS | GLY | MET | ASP | ASN | LEU | SER | ILE | ||||
| 12 | TRP | GLU | SER | GLY | ALA | ILE | LEU | LEU | HIS | LEU | ||||
| 13 | VAL | ASN | LYS | TYR | TYR | LYS | GLU | THR | GLY | ASN | ||||
| 14 | PRO | LEU | LEU | TRP | SER | ASP | ASP | LEU | ALA | ASP | ||||
| 15 | GLN | SER | GLN | ILE | ASN | ALA | TRP | LEU | PHE | PHE | ||||
| 16 | GLN | THR | SER | GLY | HIS | ALA | PRO | MET | ILE | GLY | ||||
| 17 | GLN | ALA | LEU | HIS | PHE | ARG | TYR | PHE | HIS | SER | ||||
| 18 | GLN | LYS | ILE | ALA | SER | ALA | VAL | GLU | ARG | TYR | ||||
| 19 | THR | ASP | GLU | VAL | ARG | ARG | VAL | TYR | GLY | VAL | ||||
| 20 | VAL | GLU | MET | ALA | LEU | ALA | GLU | ARG | ARG | GLU | ||||
| 21 | ALA | LEU | VAL | MET | GLU | LEU | ASP | THR | GLU | ASN | ||||
| 22 | ALA | ALA | ALA | TYR | SER | ALA | GLY | THR | THR | PRO | ||||
| 23 | MET | SER | GLN | SER | ARG | PHE | PHE | ASP | TYR | PRO | ||||
| 24 | VAL | TRP | LEU | VAL | GLY | ASP | LYS | LEU | THR | ILE | ||||
| 25 | ALA | ASP | LEU | ALA | PHE | VAL | PRO | TRP | ASN | ASN | ||||
| 26 | VAL | VAL | ASP | ARG | ILE | GLY | ILE | ASN | ILE | LYS | ||||
| 27 | ILE | GLU | PHE | PRO | GLU | VAL | TYR | LYS | TRP | THR | ||||
| 28 | LYS | HIS | MET | MET | ARG | ARG | PRO | ALA | VAL | ILE | ||||
| 29 | LYS | ALA | LEU | ARG | GLY | GLU | ARG | GLY | SER | HIS | ||||
| 30 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: Ure2p70-354, [U-100% 13C; U-100% 15N], 20 – 40 mg; KCl 75 mM; TRIS 20 mM; EGTA 1 mM; dithiothreitol 1 mM; DSS 0.5 mg; H2O 100%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D NCACB (DREAM) | sample_1 | solid | sample_conditions_1 |
| 3D N(CO)CACB | sample_1 | solid | sample_conditions_1 |
| 3D CAN(CO)CA | sample_1 | solid | sample_conditions_1 |
| 3D CCC | sample_1 | solid | sample_conditions_1 |
| 3D NCACX (DARR) | sample_1 | solid | sample_conditions_1 |
Software:
ANALYSIS v2.1.1, CCPN - chemical shift assignment
TOPSPIN, Bruker Biospin - processing
NMR spectrometers:
- Bruker Avance 850 MHz