BMRB Entry 17493

Name: Solution structure of the MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein (MGG_03307)
Published: 2011-05-06

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PDB ID: 2l9y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17493
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein (MGG_03307)

Deposition date:

2011-02-26

Original release date:

2011-05-06

Authors:

Koharudin, Leonardus; Viscomi, Arturo; Montanini, Barbara; Kershaw, Michael; Talbot, Nicholas; Ottonello, Simone; Gronenborn, Angela

Citation:

Citation: Koharudin, Leonardus; Viscomi, Arturo; Montanini, Barbara; Kershaw, Michael; Talbot, Nicholas; Ottonello, Simone; Gronenborn, Angela. "Structure-Function Analysis of a CVNH-LysM Lectin Expressed during Plant Infection by the Rice Blast Fungus Magnaporthe oryzae." Structure 19, 662-674 (2011).
PubMed: 21565701

Assembly members:

Assembly members:
entity, polymer, 167 residues, 18176.980 Da.

Natural source:

Natural source: Common Name: rice blast fungus Taxonomy ID: 318829 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Magnaporthe oryzae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GNYAGNFSGSSRDICLDGAR LRAECRRGDGGYSTSVIDLN RYLSNDNGHFRWVSGGGGGG GTATVTVQQGDTLRDIGRRF DCDFHEIARRNNIQNEDLIY PGQVLQVPTKGGSGGGAGNF WDSARDVRLVDGGKVLEAEL RYSGGWNRSRIYLDEHIGNR NGELIHC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	407
15N chemical shifts	168
1H chemical shifts	774

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MoCVNH-LysM	1

Entities:

Entity 1, MoCVNH-LysM 167 residues - 18176.980 Da.

1

GLY

ASN

TYR

ALA

GLY

ASN

PHE

SER

GLY

SER

2

SER

ARG

ASP

ILE

CYS

LEU

ASP

GLY

ALA

ARG

3

LEU

ARG

ALA

GLU

CYS

ARG

GLY

ASP

GLY

4

GLY

TYR

SER

THR

SER

VAL

ILE

ASP

LEU

ASN

5

ARG

TYR

LEU

SER

ASN

ASP

ASN

GLY

HIS

PHE

6

ARG

TRP

VAL

SER

GLY

7

GLY

THR

ALA

THR

VAL

THR

VAL

GLN

GLY

8

ASP

THR

LEU

ARG

ASP

ILE

GLY

ARG

PHE

9

ASP

CYS

ASP

PHE

HIS

GLU

ILE

ALA

ARG

10

ASN

ILE

GLN

ASN

GLU

ASP

LEU

ILE

TYR

11

PRO

GLY

GLN

VAL

LEU

GLN

VAL

PRO

THR

LYS

12

GLY

SER

GLY

ALA

GLY

ASN

PHE

13

TRP

ASP

SER

ALA

ARG

ASP

VAL

ARG

LEU

VAL

14

ASP

GLY

LYS

VAL

LEU

GLU

ALA

GLU

LEU

15

ARG

TYR

SER

GLY

TRP

ASN

ARG

SER

ARG

16

ILE

TYR

LEU

ASP

GLU

HIS

ILE

GLY

ASN

ARG

17

ASN

GLY

GLU

LEU

ILE

HIS

CYS

Samples:

sample_1: MoCVNH-LysM, [U-100% 15N], 0.5 ± 0.01 mM; H2O 90%; D2O 10%; sodium acetate25 – 50 mM; NaCl 20 mM; NaN3 3 mM

sample_2: MoCVNH-LysM, [U-100% 13C; U-100% 15N], 0.5 ± 0.01 mM; H2O 90%; D2O 10%; sodium acetate25 – 50 mM; NaCl 20 mM; NaN3 3 mM

sample_conditions_1: ionic strength: 0.020 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.020 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_2
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_2
3D H(CCO)NH	sample_2	isotropic	sample_conditions_2
3D C(CO)NH	sample_2	isotropic	sample_conditions_2
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_2
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_2
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_2
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.2.2_01, Johnson, One Moon Scientific - data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - Automatic NOE assignment

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 800 MHz
Bruker Avance 600 MHz

PDB	2L9Y
GB	EHA50335 ELQ44149 ELQ65722
REF	XP_003716654