BMRB Entry 17478

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17478
MolProbity Validation Chart

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Title:
Structure of cIAP1 CARD
Deposition date:
2011-02-21
Original release date:
2011-05-12
Authors:
Hinds, Mark; Rautureau, Gilles; Day, Catherine
Citation:

Citation: Lopez, Juanita; John, Sidonie Wicky; Tenev, Tencho; Rautureau, Gilles; Hinds, Mark; Francalanci, Floriana; Wilson, Rebecca; Broemer, Meike; Santoro, Massimo; Day, Catherine; Meier, Pascal. "CARD-Mediated Autoinhibition of cIAP1's E3 Ligase Activity Suppresses Cell Proliferation and Migration."  Mol. Cell 42, 569-583 (2011).
PubMed: 21549626

Assembly members:

Assembly members:
cIAP1-CARD, polymer, 132 residues, 15147.519 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX:4T-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cIAP1-CARD: GSHMLNAEDEKREEEKEKQA EEMASDDLSLIRKNRMALFQ QLTCVLPILDNLLKANVINK QEHDIIKQKTQIPLQARELI DTILVKGNAAANIFKNCLKE IDSTLYKNLFVDKNMKYIPT EDVSGLSLEEQL

Data sets:
Data typeCount
13C chemical shifts156
15N chemical shifts149
1H chemical shifts165

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cIAP1 CARD1

Entities:

Entity 1, cIAP1 CARD 132 residues - 15147.519 Da.

residues 435-562 of BIRC2_HUMAN (uniprot) with 4 N-terminal vector derived residues residues, numbered 431-434

1   GLYSERHISMETLEUASNALAGLUASPGLU
2   LYSARGGLUGLUGLULYSGLULYSGLNALA
3   GLUGLUMETALASERASPASPLEUSERLEU
4   ILEARGLYSASNARGMETALALEUPHEGLN
5   GLNLEUTHRCYSVALLEUPROILELEUASP
6   ASNLEULEULYSALAASNVALILEASNLYS
7   GLNGLUHISASPILEILELYSGLNLYSTHR
8   GLNILEPROLEUGLNALAARGGLULEUILE
9   ASPTHRILELEUVALLYSGLYASNALAALA
10   ALAASNILEPHELYSASNCYSLEULYSGLU
11   ILEASPSERTHRLEUTYRLYSASNLEUPHE
12   VALASPLYSASNMETLYSTYRILEPROTHR
13   GLUASPVALSERGLYLEUSERLEUGLUGLU
14   GLNLEU

Samples:

sample_1: cIAP1-CARD 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 5 mM; H2O 90%; D2O 10%

sample_2: cIAP1-CARD, [U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 5 mM; H2O 90%; D2O 10%

sample_3: cIAP1-CARD, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 50 mM; TCEP 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.140 M; pH: 5.5; pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
CC(CO)NHsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
3D HNHBsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

XEASY, Bartels et al. - data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAG10600 BAG36235 BAG64288
EMBL CAH91479
GB AAC41942 AAC50372 AAC50508 AAH16174 AAH28578
PRF 2205253A
REF NP_001157 NP_001243092 NP_001243095 NP_001248250 XP_001152534
SP Q13490
AlphaFold Q13490

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks