BMRB Entry 17476

Name: The solution structure of the ZnF UBP domain of USP33/VDU1.
Published: 2011-03-08

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17476

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of the ZnF UBP domain of USP33/VDU1.

Deposition date:

2011-02-18

Original release date:

2011-03-08

Authors:

Allen, Mark; Bycroft, Mark

Citation:

Citation: Allen, Mark; Bycroft, Mark. "The solution structure of the ZnF UBP domain of USP33/VDU1." Protein Sci. 16, 2072-2075 (2007).
PubMed: 17766394

Assembly members:

Assembly members:
zinc_finger, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSETa (modified)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zinc_finger: RNHCPHLDSVGEITKEDLIQ KSLGTCQDCKVQGPNLWACL ENRCSYVGCGESQVDHSTIH SQETKHYLTVNLTTLRVWCY ACSKEVFLDRKLGTQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	313
15N chemical shifts	89
1H chemical shifts	664

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zinc-finger	1

Entities:

Entity 1, zinc-finger 95 residues - Formula weight is not available

1

ARG

ASN

HIS

CYS

PRO

HIS

LEU

ASP

SER

VAL

2

GLY

GLU

ILE

THR

LYS

GLU

ASP

LEU

ILE

GLN

3

LYS

SER

LEU

GLY

THR

CYS

GLN

ASP

CYS

LYS

4

VAL

GLN

GLY

PRO

ASN

LEU

TRP

ALA

CYS

LEU

5

GLU

ASN

ARG

CYS

SER

TYR

VAL

GLY

CYS

GLY

6

GLU

SER

GLN

VAL

ASP

HIS

SER

THR

ILE

HIS

7

SER

GLN

GLU

THR

LYS

HIS

TYR

LEU

THR

VAL

8

ASN

LEU

THR

LEU

ARG

VAL

TRP

CYS

TYR

9

ALA

CYS

SER

LYS

GLU

VAL

PHE

LEU

ASP

ARG

10

LYS

LEU

GLY

THR

GLN

Samples:

sample_1: zinc_finger, [U-99% 13C; U-99% 15N], 1.0 mM; MES 20 mM; NaCl 50 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks