BMRB Entry 17475

Name: Structural basis of p63a SAM domain mutants involved in AEC syndrome
Published: 2011-06-01

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PDB ID: 2y9t
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17475
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural basis of p63a SAM domain mutants involved in AEC syndrome

Deposition date:

2011-02-18

Original release date:

2011-06-01

Authors:

Sathyamurthy, A.; Freund, S.; Johnson, C.; Allen, Mark

Citation:

Citation: Sathyamurthy, Aruna; Freund, Stefan; Johnson, Christopher; Allen, Mark; Bycroft, Mark. "Structural basis of p63 SAM domain mutants involved in AEC syndrome." FEBS J. 278, 2680-2688 (2011).
PubMed: 21615690

Assembly members:

Assembly members:
TUMOR_PROTEIN_63, polymer, 82 residues, 9321.517 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TUMOR_PROTEIN_63: GSYPTDCSIVSFLARLGCSS CLDYFTTQGLTTIYQIEHYS MDDLASLKIPEQFRHAIWKG ILDHRQLHEFSSPSHLLRTP SS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	157
15N chemical shifts	75
1H chemical shifts	340

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TUMOR PROTEIN 63	1

Entities:

Entity 1, TUMOR PROTEIN 63 82 residues - 9321.517 Da.

1

GLY

SER

TYR

PRO

THR

ASP

CYS

SER

ILE

VAL

2

SER

PHE

LEU

ALA

ARG

LEU

GLY

CYS

SER

3

CYS

LEU

ASP

TYR

PHE

THR

GLN

GLY

LEU

4

THR

ILE

TYR

GLN

ILE

GLU

HIS

TYR

SER

5

MET

ASP

LEU

ALA

SER

LEU

LYS

ILE

PRO

6

GLU

GLN

PHE

ARG

HIS

ALA

ILE

TRP

LYS

GLY

7

ILE

LEU

ASP

HIS

ARG

GLN

LEU

HIS

GLU

PHE

8

SER

PRO

SER

HIS

LEU

ARG

THR

PRO

9

SER

Samples:

sample_1: TUMOR PROTEIN 63, [U-13C; U-15N], 1.5 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150.000 mM; pH: 6.500; pressure: 1 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H NOESY	sample_1	solution	sample_conditions_1
2D TOCSY	sample_1	solution	sample_conditions_1
DQF COSY	sample_1	solution	sample_conditions_1
HSQC (13C and 15N)	sample_1	solution	sample_conditions_1
3D HNCACB	sample_1	solution	sample_conditions_1
CBCACONH	sample_1	solution	sample_conditions_1
CCCONH	sample_1	solution	sample_conditions_1
HNCACO	sample_1	solution	sample_conditions_1
HNCO	sample_1	solution	sample_conditions_1
HNHB	sample_1	solution	sample_conditions_1

Software:

Ansig3.3 vany, Kraulis -

AutoDep v4.3, PDBe -

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read -

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz
Bruker Avance 500 MHz

UNP	P63_HUMAN
PDB	2Y9T 2Y9U
DBJ	BAA32432 BAA32433 BAA32593 BAB20591 BAE23286
EMBL	CAA76562 CAB88216 CAC37098 CAC37099
GB	AAC43038 AAC62635 AAC62636 AAC62641 AAC62644
REF	NP_001108452 NP_001120731 NP_001120736 NP_001120814 NP_003713
SP	O88898 Q9H3D4 Q9JJP6
AlphaFold	Q9UP74 O88898 Q9H3D4 Q9JJP6