BMRB Entry 17474

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CD147 Ig0 C66M domain.
Published: 2011-06-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17474

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for CD147 Ig0 C66M domain.

Deposition date:

2011-02-17

Original release date:

2011-06-01

Authors:

Redzic, Jasmina; Armstrong, Geoffrey; Isern, Nancy; Kieft, Jeffrey; Eisenmesser, Elan

Citation:

Citation: Redzic, Jasmina; Armstrong, Geoffrey; Isern, Nancy; Jones, David; Kieft, Jeffrey; Eisenmesser, Elan Zohar. "The retinal specific CD147 Ig0 domain: from molecular structure to biological activity." J. Mol. Biol. 411, 68-82 (2011).
PubMed: 21620857

Assembly members:

Assembly members:
CD147_Ig0_C66M, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD147_Ig0_C66M: MGSSHHHHHHSSGLVPRGSH MAGFVQAPLSQQRWVGGSVE LHCEAVGSPVPEIQWWFEGQ GPNDIMSQLWDGARLDRVHI HATYHQHAASTISIDTLVEE DTGTYECRASNDPDRNHLTR APRVKWVRAQAVVLVLE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	214
15N chemical shifts	106
1H chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD147 Ig0 C66M_Molecule 1	1
2	CD147 Ig0 C66M_Molecule 2	1

Entities:

Entity 1, CD147 Ig0 C66M_Molecule 1 137 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

ALA

GLY

PHE

VAL

GLN

ALA

PRO

LEU

SER

4

GLN

ARG

TRP

VAL

GLY

SER

VAL

GLU

5

LEU

HIS

CYS

GLU

ALA

VAL

GLY

SER

PRO

VAL

6

PRO

GLU

ILE

GLN

TRP

PHE

GLU

GLY

GLN

7

GLY

PRO

ASN

ASP

ILE

MET

SER

GLN

LEU

TRP

8

ASP

GLY

ALA

ARG

LEU

ASP

ARG

VAL

HIS

ILE

9

HIS

ALA

THR

TYR

HIS

GLN

HIS

ALA

SER

10

THR

ILE

SER

ILE

ASP

THR

LEU

VAL

GLU

11

ASP

THR

GLY

THR

TYR

GLU

CYS

ARG

ALA

SER

12

ASN

ASP

PRO

ASP

ARG

ASN

HIS

LEU

THR

ARG

13

ALA

PRO

ARG

VAL

LYS

TRP

VAL

ARG

ALA

GLN

14

ALA

VAL

LEU

VAL

LEU

GLU

Samples:

sample_1: Ammonium Chloride, [U-99% 15N], 0.1 w/v; D2O, [U-99% 2H], 100%; glucose, U-13C6, 99%, 0.2 w/v; TRIS 50 mM; sodium chloride 150 mM

2H15N13C_sample: ionic strength: 0.25 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	2H15N13C_sample
3D 1H-15N NOESY	sample_1	isotropic	2H15N13C_sample
3D HNCACB	sample_1	isotropic	2H15N13C_sample
3D CBCA(CO)NH	sample_1	isotropic	2H15N13C_sample

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

Varian Uniform NMR System 900 MHz
Varian Uniform NMR System 800 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks