BMRB Entry 17472

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17472

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Title:
TRPV5 C-terminal peptide
Deposition date:
2011-02-16
Original release date:
2011-05-19
Authors:
Kovalevskaya, Nadezda
Citation:

Citation: de Groot, Theun; Kovalevskaya, Nadezda; Verkaart, Sjoerd; Schilderink, Nathalie; Felici, Marco; van der Hagen, Eline; Bindels, Rene; Vuister, Geerten; Hoenderop, Joost. "Molecular Mechanisms of Calmodulin Action on TRPV5 and Modulation by Parathyroid Hormone."  Mol. Cell. Biol. 31, 2845-2853 (2011).
PubMed: 21576356

Assembly members:

Assembly members:
trpv5, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEV2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
trpv5: GSSHRGWEILRQNTLGHLNL GLNLSEGDGEEVYHFLEHHH HHH

Data sets:
Data typeCount
13C chemical shifts39
15N chemical shifts25
1H chemical shifts43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1trpv51

Entities:

Entity 1, trpv5 43 residues - Formula weight is not available

1   GLYSERSERHISARGGLYTRPGLUILELEU
2   ARGGLNASNTHRLEUGLYHISLEUASNLEU
3   GLYLEUASNLEUSERGLUGLYASPGLYGLU
4   GLUVALTYRHISPHELEUGLUHISHISHIS
5   HISHISHIS

Samples:

sample_1: trpv5, [U-99% 13C; U-99% 15N], 150 uM; sodium azide 0.01% v/v; calcium chloride 10 mM; ammonium acetate 10 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 5.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.1.5., CCPN project - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

EMBL CAB96365 CAD32312
GB AAL04015 AAO13488 EAL23777 EAW51896 EHH52584
REF NP_062815 XP_001089988 XP_003270895 XP_003820606 XP_003896799
SP Q9NQA5
AlphaFold Q9NQA5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks