BMRB Entry 17469
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17469
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Citation: Sprangers, Remco; Gribun, Anna; Hwang, Peter; Houry, Walid; Kay, Lewis. "Quantitative NMR spectroscopy of supramolecular complexes: dynamic side pores in ClpP are important for product release." Proc. Natl. Acad. Sci. U.S.A. 102, 16678-16683 (2005).
PubMed: 16263929
Assembly members:
ClpP, polymer, 207 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: purified from the natural source Host organism: Escherichia coli Vector: pET3a
- chemical_rates
| Data type | Count |
| kinetic rates | 2 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ClpP | 1 |
Entities:
Entity 1, ClpP 207 residues - Formula weight is not available
| 1 | MET | SER | TYR | SER | GLY | GLU | ARG | ASP | ASN | PHE | ||||
| 2 | ALA | PRO | HIS | MET | ALA | LEU | VAL | PRO | MET | VAL | ||||
| 3 | ILE | GLU | GLN | THR | SER | ARG | GLY | GLU | ARG | SER | ||||
| 4 | PHE | ASP | ILE | TYR | SER | ARG | LEU | LEU | LYS | GLU | ||||
| 5 | ARG | VAL | ILE | PHE | LEU | THR | GLY | GLN | VAL | GLU | ||||
| 6 | ASP | HIS | MET | ALA | ASN | LEU | ILE | VAL | ALA | GLN | ||||
| 7 | MET | LEU | PHE | LEU | GLU | ALA | GLU | ASN | PRO | GLU | ||||
| 8 | LYS | ASP | ILE | TYR | LEU | TYR | ILE | ASN | SER | PRO | ||||
| 9 | GLY | GLY | VAL | ILE | THR | ALA | GLY | MET | SER | ILE | ||||
| 10 | TYR | ASP | THR | MET | GLN | PHE | ILE | LYS | PRO | ASP | ||||
| 11 | VAL | SER | THR | ILE | CYS | MET | GLY | GLN | ALA | ALA | ||||
| 12 | SER | MET | GLY | ALA | PHE | LEU | LEU | THR | ALA | GLY | ||||
| 13 | ALA | LYS | GLY | LYS | ARG | PHE | CYS | LEU | PRO | ASN | ||||
| 14 | SER | ARG | VAL | MET | ILE | HIS | GLN | PRO | LEU | GLY | ||||
| 15 | GLY | TYR | GLN | GLY | GLN | ALA | THR | ASP | ILE | GLU | ||||
| 16 | ILE | HIS | ALA | ARG | GLU | ILE | LEU | LYS | VAL | LYS | ||||
| 17 | GLY | ARG | MET | ASN | GLU | LEU | MET | ALA | LEU | HIS | ||||
| 18 | THR | GLY | GLN | SER | LEU | GLU | GLN | ILE | GLU | ARG | ||||
| 19 | ASP | THR | GLU | ARG | ASP | ARG | PHE | LEU | SER | ALA | ||||
| 20 | PRO | GLU | ALA | VAL | GLU | TYR | GLY | LEU | VAL | ASP | ||||
| 21 | SER | ILE | LEU | THR | HIS | ARG | ASN |
Samples:
sample_1: ClpP0.3 – 0.5 mM; potassium phosphate 50 mM; DTT 1 mM; sodium azide 0.03%; D2O 100%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 273.65 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Methyl TROSY relaxation dispersion | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA94668 BAB33914 BAE76217 BAG75987 BAH62053 |
| EMBL | CAD08907 CAP74971 CAQ30909 CAQ90075 CAQ97313 |
| GB | AAA23588 AAB40193 AAC73540 AAG54787 AAL19403 |
| PIR | AC0558 |
| REF | NP_308518 NP_414971 NP_455045 NP_459444 NP_706331 |
| SP | A1A8A6 A7ZIJ5 A7ZX95 B1J011 B4SWU1 |
| AlphaFold | A1A8A6 A7ZIJ5 A7ZX95 B1J011 B4SWU1 |