BMRB Entry 17466

Name: Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)
Published: 2011-12-12

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17466

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)

Deposition date:

2011-02-11

Original release date:

2011-12-12

Authors:

Kappo, M.; Ab, E.; Atkinson, R.; Faro, A.; Muleya, V.; Mulaudzi, T.; Poole, J.; Mckenzie, J.; PUGH, DAVID

Citation:

Citation: Kappo, Mautin; Ab, Eiso; Hassem, Faqeer; Atkinson, R. Andrew; Faro, Andrew; Muleya, Victor; Mulaudzi, Takalani; Poole, John; McKenzie, Jean; Chibi, Moredreck; Moolman-Smook, Joanna; Rees, D. Jasper G; Pugh, David. "Solution Structure of RING Finger-like Domain of Retinoblastoma-binding Protein-6 (RBBP6) Suggests It Functions as a U-box." J. Biol. Chem. 287, 7146-7158 (2012).
PubMed: 22130672

Assembly members:

Assembly members:
E3_UBIQUITIN-PROTEIN_LIGASE_RBBP6, polymer, 92 residues, 10214.4517 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E3_UBIQUITIN-PROTEIN_LIGASE_RBBP6: GPLGSEDDPIPDELLCLICK DIMTDAVVIPCCGNSYCDEC IRTALLESDEHTCPTCHQND VSPDALIANKFLRQAVNNFK NETGYTKRLRKQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	300
15N chemical shifts	96
1H chemical shifts	631

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E3 UBIQUITIN-PROTEIN LIGASE RBBP6, 1	1
2	E3 UBIQUITIN-PROTEIN LIGASE RBBP6, 2	1
3	ZINC_ION, 1	2
4	ZINC_ION, 2	2

Entities:

Entity 1, E3 UBIQUITIN-PROTEIN LIGASE RBBP6, 1 92 residues - 10214.4517 Da.

1

GLY

PRO

LEU

GLY

SER

GLU

ASP

PRO

ILE

2

PRO

ASP

GLU

LEU

CYS

LEU

ILE

CYS

LYS

3

ASP

ILE

MET

THR

ASP

ALA

VAL

ILE

PRO

4

CYS

GLY

ASN

SER

TYR

CYS

ASP

GLU

CYS

5

ILE

ARG

THR

ALA

LEU

GLU

SER

ASP

GLU

6

HIS

THR

CYS

PRO

THR

CYS

HIS

GLN

ASN

ASP

7

VAL

SER

PRO

ASP

ALA

LEU

ILE

ALA

ASN

LYS

8

PHE

LEU

ARG

GLN

ALA

VAL

ASN

PHE

LYS

9

ASN

GLU

THR

GLY

TYR

THR

LYS

ARG

LEU

ARG

10

LYS

GLN

Entity 2, ZINC_ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: E3 UBIQUITIN-PROTEIN LIGASE RBBP6 0.7 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC-NOESY	sample_1	solution	sample_conditions_1
13C-HSQC-NOESY (aromatic)	sample_1	solution	sample_conditions_1
13C-HSQC-NOESY(aliphatic)	sample_1	solution	sample_conditions_1
1H-NOESY	sample_1	solution	sample_conditions_1
13C-HSQC- NOESY (AROMATIC)	sample_1	solution	sample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS v2.1, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - data analysis

NMRView vany, Johnson, One Moon Scientific - chemical shift assignment

SPARKY vany, Goddard - chemical shift assignment

NMR spectrometers:

Varian UnityInova 600 MHz

UNP	RBBP6_HUMAN
BMRB	11159 17772
PDB	2YUR 3ZTG
DBJ	BAC77636 BAC77637 BAG64162
EMBL	CAA59445
GB	AAB49620 AAC72432 AAH63524 AAI72357 AAL05625
REF	NP_001291483 NP_008841 NP_035377 NP_061173 XP_001076339
SP	P97868 Q7Z6E9
TPG	DAA15466
AlphaFold	Q9NPX4 P97868 Q7Z6E9