BMRB Entry 17465

Title:
Structural Characterisation of Apoflavodoxin shows that the Location of the Stable Nucleus Differs Among Proteins with a Flavodoxin-like Topology
Deposition date:
2011-02-11
Original release date:
2016-06-09
Authors:
Steensma, Elles; van Mierlo, Carlo
Citation:

Citation: Steensma, Elles; van Mierlo, Carlo. "Structural Characterisation of Apoflavodoxin shows that the Location of the Stable Nucleus Differs Among Proteins with a Flavodoxin-like Topology"  J. Mol. Biol. 282, 653-666 (1998).

Assembly members:

Assembly members:
aII, polymer, 179 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: A. vinelandii   Taxonomy ID: 354   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Azotobacter vinelandii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ns

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aII1

Entities:

Entity 1, aII 179 residues - Formula weight is not available

1   ALALYSILEGLYLEUPHEPHEGLYSERASN
2   THRGLYLYSTHRARGLYSVALALALYSSER
3   ILELYSLYSARGPHEASPASPGLUTHRMET
4   SERASPALALEUASNVALASNARGVALSER
5   ALAGLUASPPHEALAGLNTYRGLNPHELEU
6   ILELEUGLYTHRPROTHRLEUGLYGLUGLY
7   GLULEUPROGLYLEUSERSERASPALAGLU
8   ASNGLUSERTRPGLUGLUPHELEUPROLYS
9   ILEGLUGLYLEUASPPHESERGLYLYSTHR
10   VALALALEUPHEGLYLEUGLYASPGLNVAL
11   GLYTYRPROGLUASNTYRLEUASPALALEU
12   GLYGLULEUTYRSERPHEPHELYSASPARG
13   GLYALALYSILEVALGLYSERTRPSERTHR
14   ASPGLYTYRGLUPHEGLUSERSERGLUALA
15   VALVALASPGLYLYSPHEVALGLYLEUALA
16   LEUASPLEUASPASNGLNSERGLYLYSTHR
17   ASPGLUARGVALALAALATRPLEUALAGLN
18   ILEALAPROGLUPHEGLYLEUSERLEU

Samples:

sample_1: aII, [U-15N], 2 mM; potassium pyrophosphate 150 mM; D2O 100%

sample_conditions_1: pH*: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

FELIX v2.5, Accelrys Software Inc. - processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 4886