BMRB Entry 17463
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17463
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Walters, Kylie; Gassner, George; Lippard, Stephen; Wagner, Gerhard. "Structure of the soluble methane monooxygenase regulatory protein B" Proc. Natl. Acad. Sci. U.S.A. 96, 7877-7882 (1999).
Assembly members:
MMOB, polymer, 141 residues, Formula weight is not available
MMOBHa, polymer, 512 residues, Formula weight is not available
MMOBHb, polymer, 384 residues, Formula weight is not available
MMOBHg, polymer, 162 residues, Formula weight is not available
Natural source: Common Name: M. capsulatus Taxonomy ID: 414 Superkingdom: Bacteria Kingdom: not available Genus/species: Methylococcus capsulatus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
MMOB: MSVNSNAYDAGIMGLKGKDF
ADQFFADENQVVHESDTVVL
VLKKSDEINTFIEEILLTDY
KKNVNPTVNVEDRAGYWWIK
ANGKIEVDCDEISELLGRQF
NVYDFLVDVSSTIGRAYTLG
NKFTITSELMGLDRKLEDYH
A
MMOBHa: AANRAPTSVNAQEVHRWLQS
FNWDFKNNRTKYATKYKMAN
ETKEQFKLIAKEYARMEAVK
DERQFGSLQVALTRLNAGVR
VHPKWNETMKVVSNFLEVGE
YNAIAATGMLWDSAQAAEQK
NGYLAQVLDEIRHTHQCAYV
NYYFAKNGQDPAGHNDARRT
RTIGPLWKGMKRVFSDGFIS
GDAVECSLNLQLVGEACFTN
PLIVAVTEWAAANGDEITPT
VFLSIETDELRHMANGYQTV
VSIANDPASAKYLNTDLNNA
FWTQQKYFTPVLGMLFEYGS
KFKVEPWVKTWDRWVYEDWG
GIWIGRLGKYGVESPRSLKD
AKQDAYWAHHDLYLLAYALW
PTGFFRLALPDQEEMEWFEA
NYPGWYDHYGKIYEEWRARG
CEDPSSGFIPLMWFIENNHP
IYIDRVSQVPFCPSLAKGAS
TLRVHEYNGEMHTFSDQWGE
RMWLAEPERYECQNIFEQYE
GRELSEVIAELHGLRSDGKT
LIAQPHVRGDKLWTLDDIKR
LNCVFKNPVKAF
MMOBHb: ERRRGLTDPEMAAVILKALP
EAPLDGNNKMGYFVTPRWKR
LTEYEALTVYAQPNADWIAG
GLDWGDWTQKFHGGRPSWGN
ETTELRTVDWFKHRDPLRRW
HAPYVKDKAEEWRYTDRFLQ
GYSADGQIRAMNPTWRTSSC
NRYWGAFLFNEYGLFNAHSQ
GAREALSDVTRVSLAFWGFD
KIDIAQMIQLERGFLAKIVP
GFDESTAVPKAEWTNGEVYK
SARLAVEGLWQEVFDWNESA
FSVHAVYDALFGQFVRREFF
QRLAPRFGDNLTPFFINQAQ
TYFQIAKQGVQDLYYNCLGD
DPEFSDYNRTVMRNWTGKWL
EPTIAALRDFMGLFAKLPAG
TTDKEEITASLYRVVDDWIE
DYASAIDFKADRDQIVKAVL
AGLK
MMOBHg: LGIHSNDTRDAWVNKIAHVN
TLEKAAEMLKQFRMDHTTPF
RNSYELDNDYLWIEAKLEEK
VAVLKARAFNEVDFRHKTAF
GEDAKSVLDGTVAKMNAAKD
KWEAEKIHIGFRQAYKPPIM
PVNYFLDGERQLGTRLMELR
NLNYYDTPLEELRKQRGVRV
VH
- chemical_rates
| Data type | Count |
| kinetic rates | 2 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MMOB | 1 |
| 2 | MMOBHa | 2 |
| 3 | MMOBHb | 3 |
| 4 | MMOBHg | 4 |
Entities:
Entity 1, MMOB 141 residues - Formula weight is not available
| 1 | MET | SER | VAL | ASN | SER | ASN | ALA | TYR | ASP | ALA | ||||
| 2 | GLY | ILE | MET | GLY | LEU | LYS | GLY | LYS | ASP | PHE | ||||
| 3 | ALA | ASP | GLN | PHE | PHE | ALA | ASP | GLU | ASN | GLN | ||||
| 4 | VAL | VAL | HIS | GLU | SER | ASP | THR | VAL | VAL | LEU | ||||
| 5 | VAL | LEU | LYS | LYS | SER | ASP | GLU | ILE | ASN | THR | ||||
| 6 | PHE | ILE | GLU | GLU | ILE | LEU | LEU | THR | ASP | TYR | ||||
| 7 | LYS | LYS | ASN | VAL | ASN | PRO | THR | VAL | ASN | VAL | ||||
| 8 | GLU | ASP | ARG | ALA | GLY | TYR | TRP | TRP | ILE | LYS | ||||
| 9 | ALA | ASN | GLY | LYS | ILE | GLU | VAL | ASP | CYS | ASP | ||||
| 10 | GLU | ILE | SER | GLU | LEU | LEU | GLY | ARG | GLN | PHE | ||||
| 11 | ASN | VAL | TYR | ASP | PHE | LEU | VAL | ASP | VAL | SER | ||||
| 12 | SER | THR | ILE | GLY | ARG | ALA | TYR | THR | LEU | GLY | ||||
| 13 | ASN | LYS | PHE | THR | ILE | THR | SER | GLU | LEU | MET | ||||
| 14 | GLY | LEU | ASP | ARG | LYS | LEU | GLU | ASP | TYR | HIS | ||||
| 15 | ALA |
Entity 2, MMOBHa 512 residues - Formula weight is not available
| 1 | ALA | ALA | ASN | ARG | ALA | PRO | THR | SER | VAL | ASN | ||||
| 2 | ALA | GLN | GLU | VAL | HIS | ARG | TRP | LEU | GLN | SER | ||||
| 3 | PHE | ASN | TRP | ASP | PHE | LYS | ASN | ASN | ARG | THR | ||||
| 4 | LYS | TYR | ALA | THR | LYS | TYR | LYS | MET | ALA | ASN | ||||
| 5 | GLU | THR | LYS | GLU | GLN | PHE | LYS | LEU | ILE | ALA | ||||
| 6 | LYS | GLU | TYR | ALA | ARG | MET | GLU | ALA | VAL | LYS | ||||
| 7 | ASP | GLU | ARG | GLN | PHE | GLY | SER | LEU | GLN | VAL | ||||
| 8 | ALA | LEU | THR | ARG | LEU | ASN | ALA | GLY | VAL | ARG | ||||
| 9 | VAL | HIS | PRO | LYS | TRP | ASN | GLU | THR | MET | LYS | ||||
| 10 | VAL | VAL | SER | ASN | PHE | LEU | GLU | VAL | GLY | GLU | ||||
| 11 | TYR | ASN | ALA | ILE | ALA | ALA | THR | GLY | MET | LEU | ||||
| 12 | TRP | ASP | SER | ALA | GLN | ALA | ALA | GLU | GLN | LYS | ||||
| 13 | ASN | GLY | TYR | LEU | ALA | GLN | VAL | LEU | ASP | GLU | ||||
| 14 | ILE | ARG | HIS | THR | HIS | GLN | CYS | ALA | TYR | VAL | ||||
| 15 | ASN | TYR | TYR | PHE | ALA | LYS | ASN | GLY | GLN | ASP | ||||
| 16 | PRO | ALA | GLY | HIS | ASN | ASP | ALA | ARG | ARG | THR | ||||
| 17 | ARG | THR | ILE | GLY | PRO | LEU | TRP | LYS | GLY | MET | ||||
| 18 | LYS | ARG | VAL | PHE | SER | ASP | GLY | PHE | ILE | SER | ||||
| 19 | GLY | ASP | ALA | VAL | GLU | CYS | SER | LEU | ASN | LEU | ||||
| 20 | GLN | LEU | VAL | GLY | GLU | ALA | CYS | PHE | THR | ASN | ||||
| 21 | PRO | LEU | ILE | VAL | ALA | VAL | THR | GLU | TRP | ALA | ||||
| 22 | ALA | ALA | ASN | GLY | ASP | GLU | ILE | THR | PRO | THR | ||||
| 23 | VAL | PHE | LEU | SER | ILE | GLU | THR | ASP | GLU | LEU | ||||
| 24 | ARG | HIS | MET | ALA | ASN | GLY | TYR | GLN | THR | VAL | ||||
| 25 | VAL | SER | ILE | ALA | ASN | ASP | PRO | ALA | SER | ALA | ||||
| 26 | LYS | TYR | LEU | ASN | THR | ASP | LEU | ASN | ASN | ALA | ||||
| 27 | PHE | TRP | THR | GLN | GLN | LYS | TYR | PHE | THR | PRO | ||||
| 28 | VAL | LEU | GLY | MET | LEU | PHE | GLU | TYR | GLY | SER | ||||
| 29 | LYS | PHE | LYS | VAL | GLU | PRO | TRP | VAL | LYS | THR | ||||
| 30 | TRP | ASP | ARG | TRP | VAL | TYR | GLU | ASP | TRP | GLY | ||||
| 31 | GLY | ILE | TRP | ILE | GLY | ARG | LEU | GLY | LYS | TYR | ||||
| 32 | GLY | VAL | GLU | SER | PRO | ARG | SER | LEU | LYS | ASP | ||||
| 33 | ALA | LYS | GLN | ASP | ALA | TYR | TRP | ALA | HIS | HIS | ||||
| 34 | ASP | LEU | TYR | LEU | LEU | ALA | TYR | ALA | LEU | TRP | ||||
| 35 | PRO | THR | GLY | PHE | PHE | ARG | LEU | ALA | LEU | PRO | ||||
| 36 | ASP | GLN | GLU | GLU | MET | GLU | TRP | PHE | GLU | ALA | ||||
| 37 | ASN | TYR | PRO | GLY | TRP | TYR | ASP | HIS | TYR | GLY | ||||
| 38 | LYS | ILE | TYR | GLU | GLU | TRP | ARG | ALA | ARG | GLY | ||||
| 39 | CYS | GLU | ASP | PRO | SER | SER | GLY | PHE | ILE | PRO | ||||
| 40 | LEU | MET | TRP | PHE | ILE | GLU | ASN | ASN | HIS | PRO | ||||
| 41 | ILE | TYR | ILE | ASP | ARG | VAL | SER | GLN | VAL | PRO | ||||
| 42 | PHE | CYS | PRO | SER | LEU | ALA | LYS | GLY | ALA | SER | ||||
| 43 | THR | LEU | ARG | VAL | HIS | GLU | TYR | ASN | GLY | GLU | ||||
| 44 | MET | HIS | THR | PHE | SER | ASP | GLN | TRP | GLY | GLU | ||||
| 45 | ARG | MET | TRP | LEU | ALA | GLU | PRO | GLU | ARG | TYR | ||||
| 46 | GLU | CYS | GLN | ASN | ILE | PHE | GLU | GLN | TYR | GLU | ||||
| 47 | GLY | ARG | GLU | LEU | SER | GLU | VAL | ILE | ALA | GLU | ||||
| 48 | LEU | HIS | GLY | LEU | ARG | SER | ASP | GLY | LYS | THR | ||||
| 49 | LEU | ILE | ALA | GLN | PRO | HIS | VAL | ARG | GLY | ASP | ||||
| 50 | LYS | LEU | TRP | THR | LEU | ASP | ASP | ILE | LYS | ARG | ||||
| 51 | LEU | ASN | CYS | VAL | PHE | LYS | ASN | PRO | VAL | LYS | ||||
| 52 | ALA | PHE |
Entity 3, MMOBHb 384 residues - Formula weight is not available
| 1 | GLU | ARG | ARG | ARG | GLY | LEU | THR | ASP | PRO | GLU | ||||
| 2 | MET | ALA | ALA | VAL | ILE | LEU | LYS | ALA | LEU | PRO | ||||
| 3 | GLU | ALA | PRO | LEU | ASP | GLY | ASN | ASN | LYS | MET | ||||
| 4 | GLY | TYR | PHE | VAL | THR | PRO | ARG | TRP | LYS | ARG | ||||
| 5 | LEU | THR | GLU | TYR | GLU | ALA | LEU | THR | VAL | TYR | ||||
| 6 | ALA | GLN | PRO | ASN | ALA | ASP | TRP | ILE | ALA | GLY | ||||
| 7 | GLY | LEU | ASP | TRP | GLY | ASP | TRP | THR | GLN | LYS | ||||
| 8 | PHE | HIS | GLY | GLY | ARG | PRO | SER | TRP | GLY | ASN | ||||
| 9 | GLU | THR | THR | GLU | LEU | ARG | THR | VAL | ASP | TRP | ||||
| 10 | PHE | LYS | HIS | ARG | ASP | PRO | LEU | ARG | ARG | TRP | ||||
| 11 | HIS | ALA | PRO | TYR | VAL | LYS | ASP | LYS | ALA | GLU | ||||
| 12 | GLU | TRP | ARG | TYR | THR | ASP | ARG | PHE | LEU | GLN | ||||
| 13 | GLY | TYR | SER | ALA | ASP | GLY | GLN | ILE | ARG | ALA | ||||
| 14 | MET | ASN | PRO | THR | TRP | ARG | THR | SER | SER | CYS | ||||
| 15 | ASN | ARG | TYR | TRP | GLY | ALA | PHE | LEU | PHE | ASN | ||||
| 16 | GLU | TYR | GLY | LEU | PHE | ASN | ALA | HIS | SER | GLN | ||||
| 17 | GLY | ALA | ARG | GLU | ALA | LEU | SER | ASP | VAL | THR | ||||
| 18 | ARG | VAL | SER | LEU | ALA | PHE | TRP | GLY | PHE | ASP | ||||
| 19 | LYS | ILE | ASP | ILE | ALA | GLN | MET | ILE | GLN | LEU | ||||
| 20 | GLU | ARG | GLY | PHE | LEU | ALA | LYS | ILE | VAL | PRO | ||||
| 21 | GLY | PHE | ASP | GLU | SER | THR | ALA | VAL | PRO | LYS | ||||
| 22 | ALA | GLU | TRP | THR | ASN | GLY | GLU | VAL | TYR | LYS | ||||
| 23 | SER | ALA | ARG | LEU | ALA | VAL | GLU | GLY | LEU | TRP | ||||
| 24 | GLN | GLU | VAL | PHE | ASP | TRP | ASN | GLU | SER | ALA | ||||
| 25 | PHE | SER | VAL | HIS | ALA | VAL | TYR | ASP | ALA | LEU | ||||
| 26 | PHE | GLY | GLN | PHE | VAL | ARG | ARG | GLU | PHE | PHE | ||||
| 27 | GLN | ARG | LEU | ALA | PRO | ARG | PHE | GLY | ASP | ASN | ||||
| 28 | LEU | THR | PRO | PHE | PHE | ILE | ASN | GLN | ALA | GLN | ||||
| 29 | THR | TYR | PHE | GLN | ILE | ALA | LYS | GLN | GLY | VAL | ||||
| 30 | GLN | ASP | LEU | TYR | TYR | ASN | CYS | LEU | GLY | ASP | ||||
| 31 | ASP | PRO | GLU | PHE | SER | ASP | TYR | ASN | ARG | THR | ||||
| 32 | VAL | MET | ARG | ASN | TRP | THR | GLY | LYS | TRP | LEU | ||||
| 33 | GLU | PRO | THR | ILE | ALA | ALA | LEU | ARG | ASP | PHE | ||||
| 34 | MET | GLY | LEU | PHE | ALA | LYS | LEU | PRO | ALA | GLY | ||||
| 35 | THR | THR | ASP | LYS | GLU | GLU | ILE | THR | ALA | SER | ||||
| 36 | LEU | TYR | ARG | VAL | VAL | ASP | ASP | TRP | ILE | GLU | ||||
| 37 | ASP | TYR | ALA | SER | ALA | ILE | ASP | PHE | LYS | ALA | ||||
| 38 | ASP | ARG | ASP | GLN | ILE | VAL | LYS | ALA | VAL | LEU | ||||
| 39 | ALA | GLY | LEU | LYS |
Entity 4, MMOBHg 162 residues - Formula weight is not available
| 1 | LEU | GLY | ILE | HIS | SER | ASN | ASP | THR | ARG | ASP | ||||
| 2 | ALA | TRP | VAL | ASN | LYS | ILE | ALA | HIS | VAL | ASN | ||||
| 3 | THR | LEU | GLU | LYS | ALA | ALA | GLU | MET | LEU | LYS | ||||
| 4 | GLN | PHE | ARG | MET | ASP | HIS | THR | THR | PRO | PHE | ||||
| 5 | ARG | ASN | SER | TYR | GLU | LEU | ASP | ASN | ASP | TYR | ||||
| 6 | LEU | TRP | ILE | GLU | ALA | LYS | LEU | GLU | GLU | LYS | ||||
| 7 | VAL | ALA | VAL | LEU | LYS | ALA | ARG | ALA | PHE | ASN | ||||
| 8 | GLU | VAL | ASP | PHE | ARG | HIS | LYS | THR | ALA | PHE | ||||
| 9 | GLY | GLU | ASP | ALA | LYS | SER | VAL | LEU | ASP | GLY | ||||
| 10 | THR | VAL | ALA | LYS | MET | ASN | ALA | ALA | LYS | ASP | ||||
| 11 | LYS | TRP | GLU | ALA | GLU | LYS | ILE | HIS | ILE | GLY | ||||
| 12 | PHE | ARG | GLN | ALA | TYR | LYS | PRO | PRO | ILE | MET | ||||
| 13 | PRO | VAL | ASN | TYR | PHE | LEU | ASP | GLY | GLU | ARG | ||||
| 14 | GLN | LEU | GLY | THR | ARG | LEU | MET | GLU | LEU | ARG | ||||
| 15 | ASN | LEU | ASN | TYR | TYR | ASP | THR | PRO | LEU | GLU | ||||
| 16 | GLU | LEU | ARG | LYS | GLN | ARG | GLY | VAL | ARG | VAL | ||||
| 17 | VAL | HIS |
Samples:
sample_1: sodium phosphate buffer 50 mM; Pefabloc 4 mM; MMOB, [U-15N], 0.2 mM; sodium azide 0.1%; MMOBHa0.02 – 0.1 mM; MMOBb0.02 – 0.1 mM; MMOBg0.02 – 0.1 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_2 |
Software:
FELIX, Accelrys Software Inc. - processing
XEASY, Bartels et al. - data analysis
NMR spectrometers:
- Varian INOVA 750 MHz
- Bruker DMX 600 MHz
- Varian Unity 500 MHz
- Varian INOVA 500 MHz
- Varian UnityPlus 400 MHz