BMRB Entry 17456

Name: 1H, 15N, 13C chemical shift assignment of the THAP domain 1-81 from the cell growth suppressor human THAP11 protein
Published: 2012-09-24

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PDB ID: 2lau
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17456
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C chemical shift assignment of the THAP domain 1-81 from the cell growth suppressor human THAP11 protein

Deposition date:

2011-02-11

Original release date:

2012-09-24

Authors:

Durand, Jade; Milon, Alain; Gervais, Virginie

Citation:

Citation: Gervais, Virginie; Campagne, Sebastien; Durand, Jade; Muller, Isabelle; Milon, Alain. "NMR studies of a new family of DNA binding proteins: the THAP proteins." J. Biomol. NMR 56, 3-15 (2013).
PubMed: 23306615

Assembly members:

Assembly members:
THAP_domain, polymer, 81 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
THAP_domain: GSPGFTCCVPGCYNNSHRDK ALHFYTFPKDAELRRLWLKN VSRAGVSGCFSTFQPTTGHR LCSVHFQGGRKTYTVRVPTI F

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	342
15N chemical shifts	76
1H chemical shifts	530

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	THAP domain of human THAP11	1
2	ZINC ION	2

Entities:

Entity 1, THAP domain of human THAP11 81 residues - Formula weight is not available

1

GLY

SER

PRO

GLY

PHE

THR

CYS

VAL

PRO

2

GLY

CYS

TYR

ASN

SER

HIS

ARG

ASP

LYS

3

ALA

LEU

HIS

PHE

TYR

THR

PHE

PRO

LYS

ASP

4

ALA

GLU

LEU

ARG

LEU

TRP

LEU

LYS

ASN

5

VAL

SER

ARG

ALA

GLY

VAL

SER

GLY

CYS

PHE

6

SER

THR

PHE

GLN

PRO

THR

GLY

HIS

ARG

7

LEU

CYS

SER

VAL

HIS

PHE

GLN

GLY

ARG

8

LYS

THR

TYR

THR

VAL

ARG

VAL

PRO

THR

ILE

9

PHE

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: THAP domain, [U-99% 13C; U-99% 15N], 0,5 mM; Tris-HCl 50 mM; sodium chloride 30 mM; DTT 5 mM

sample_2: THAP domain 0,7 mM; Tris-HCl 50 mM; sodium chloride 30 mM; DTT 5 mM

sample_3: THAP domain, [U-99% 15N], 0,7 mM; Tris-HCl 50 mM; sodium chloride 30 mM; DTT 5 mM

sample_conditions_1: ionic strength: 30 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking, processing

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 950 MHz
Bruker Avance 600 MHz

SP	Q96EK4 A5PKF5 Q96EK4 Q9JJD0
PDB	2LAU
DBJ	BAA95063 BAE38498 BAE41810 BAF82056
GB	AAH12182 AAI38967 AAI38988 AAI42470 ABM83526
REF	NP_001098464 NP_001100892 NP_001233111 NP_065190 NP_067488
TPG	DAA20120
AlphaFold	O94795 A5PKF5 Q96EK4 Q9JJD0