BMRB Entry 17450
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17450
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Citation: Unnerstale, Sofia; Maler, Lena; Draheim, Roger. "Structural characterization of AS1-membrane interactions from a subset of HAMP domains." Biochim. Biophys. Acta. 1808, 2403-2412 (2011).
PubMed: 21763270
Assembly members:
AS1p-TarEc, polymer, 19 residues, 2120.664 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: chemical synthesis Host organism: Escherichia coli
Entity Sequences (FASTA):
AS1p-TarEc: RMLLTPLAKIIAHIREIAG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 116 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AS1p-TarEc | 1 |
Entities:
Entity 1, AS1p-TarEc 19 residues - 2120.664 Da.
| 1 | ARG | MET | LEU | LEU | THR | PRO | LEU | ALA | LYS | ILE | ||||
| 2 | ILE | ALA | HIS | ILE | ARG | GLU | ILE | ALA | GLY |
Samples:
sample_1: sodium phosphate 50 mM; DHPC, [U-2H], 240 mM; DMPC, [U-2H], 54 mM; DMPG, [U-2H], 6 mM; H2O 90%; D2O 10%; AS1p-TarEc 2 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection
SPARKY v3.114, Goddard - chemical shift assignment, peak picking
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz