BMRB Entry 17435

Name: solution structure of the C-terminal domain of Salmonella H-NS
Published: 2015-06-23

Click here to enlarge.

PDB ID: 2l93
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17435
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

solution structure of the C-terminal domain of Salmonella H-NS

Deposition date:

2011-01-29

Original release date:

2015-06-23

Authors:

Li, Yifei

Citation:

Citation: Gordon, Blair; Li, Yifei; Cote, Atina; Weirauch, Matthew; Ding, Pengfei; Hughes, Timothy; Navarre, William; Xia, Bin; Liu, Jun. "Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins" Proc. Natl. Acad. Sci. U. S. A. 108, 10690-10695 (2011).
PubMed: 21673140

Assembly members:

Assembly members:
H-NS, polymer, 49 residues, 5564.294 Da.

Natural source:

Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 90371 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella typhimurium

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H-NS: AARPAKYSYVDENGETKTWT GQGRTPAVIKKAMEEQGKQL EDFLIKELE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	173
15N chemical shifts	51
1H chemical shifts	350

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	H-NS	1

Entities:

Entity 1, H-NS 49 residues - 5564.294 Da.

1

ALA

ARG

PRO

ALA

LYS

TYR

SER

TYR

VAL

2

ASP

GLU

ASN

GLY

GLU

THR

LYS

THR

TRP

THR

3

GLY

GLN

GLY

ARG

THR

PRO

ALA

VAL

ILE

LYS

4

LYS

ALA

MET

GLU

GLN

GLY

LYS

GLN

LEU

5

GLU

ASP

PHE

LEU

ILE

LYS

GLU

LEU

GLU

Samples:

sample_1: C-terminal domain of Salmonella H-NS, [U-13C, >90%; U-15 N, >90%], 0.7 mM; sodium phosphate, [U-100% 13C; U-100% 15N], 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - geometry optimization

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2L93
DBJ	BAJ36716 BAP07615 GAL42187 GAL43811 GAL48349
EMBL	CAA32549 CAD08382 CAR32863 CAR37239 CAR59200
GB	AAB61148 AAL20669 AAO69288 AAV77088 AAX65652
PIR	AC0650
REF	NP_455749 NP_460710 WP_001287382 WP_001287383 WP_001748450
SP	P0A1S2 P0A1S3 Q5PCT5
AlphaFold	P0A1S2 P0A1S3 Q5PCT5