BMRB Entry 17428

Name: AIDA1 PTB domain
Published: 2012-08-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17428

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

AIDA1 PTB domain

Deposition date:

2011-01-25

Original release date:

2012-08-14

Authors:

Donaldson, Logan

Citation:

Citation: Donaldson, Logan. "Improving protein solubility by aromatic ablation" J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
AIDA1, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJExpress405-kan

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AIDA1: HHHHHHLVPRGSGYENPTYK FFETLRPPNEATALQTPVQA WQHHPEKLIAQSCDYKAAYL GSMLIKELRGTESTQDACAK MRANCQKSTEQMKKVPTIIL SVSAKGVKFIDATNKNIIAE HEIRNISCAAQDPEDLSTFA YITKDLKSNHHYCHVFTAFD VNLAAEIILTLGQAFEVAYQ LALQARKLE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	568
15N chemical shifts	134
1H chemical shifts	860

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PTB domain	1

Entities:

Entity 1, PTB domain 189 residues - Formula weight is not available

1

HIS

LEU

VAL

PRO

ARG

2

GLY

SER

GLY

TYR

GLU

ASN

PRO

THR

TYR

LYS

3

PHE

GLU

THR

LEU

ARG

PRO

ASN

GLU

4

ALA

THR

ALA

LEU

GLN

THR

PRO

VAL

GLN

ALA

5

TRP

GLN

HIS

PRO

GLU

LYS

LEU

ILE

ALA

6

GLN

SER

CYS

ASP

TYR

LYS

ALA

TYR

LEU

7

GLY

SER

MET

LEU

ILE

LYS

GLU

LEU

ARG

GLY

8

THR

GLU

SER

THR

GLN

ASP

ALA

CYS

ALA

LYS

9

MET

ARG

ALA

ASN

CYS

GLN

LYS

SER

THR

GLU

10

GLN

MET

LYS

VAL

PRO

THR

ILE

LEU

11

SER

VAL

SER

ALA

LYS

GLY

VAL

LYS

PHE

ILE

12

ASP

ALA

THR

ASN

LYS

ASN

ILE

ALA

GLU

13

HIS

GLU

ILE

ARG

ASN

ILE

SER

CYS

ALA

14

GLN

ASP

PRO

GLU

ASP

LEU

SER

THR

PHE

ALA

15

TYR

ILE

THR

LYS

ASP

LEU

LYS

SER

ASN

HIS

16

HIS

TYR

CYS

HIS

VAL

PHE

THR

ALA

PHE

ASP

17

VAL

ASN

LEU

ALA

GLU

ILE

LEU

THR

18

LEU

GLY

GLN

ALA

PHE

GLU

VAL

ALA

TYR

GLN

19

LEU

ALA

LEU

GLN

ALA

ARG

LYS

LEU

GLU

Samples:

sample_1: AIDA1, [U-99% 13C; U-99% 15N], 0.6 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.8; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 900 MHz
Varian NMRS 600 MHz

BMRB	17934
PDB	2M38
DBJ	BAE87950
GB	EDL21516 EDL21517 EDL21518 EDM16964 EDM16965
REF	XP_003126745 XP_005658157 XP_006077488 XP_013846696