BMRB Entry 17427

Name: Chemical Shift Assignments for p21-KID Bound to Cdk2 and Cyclin A in Solution
Published: 2011-03-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17427

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignments for p21-KID Bound to Cdk2 and Cyclin A in Solution

Deposition date:

2011-01-25

Original release date:

2011-03-08

Authors:

Wang, Yuefeng; Fisher, John; Mattew, Rose; Ou, Li; Otieno, Steve; Sublett, Jack; Xiao, Limin; Chen, Jianhan; Roussel, Martine; Kriwacki, Richard

Citation:

Citation: Wang, Yuefeng; Fisher, John; Mathew, Rose; Ou, Li; Otieno, Steve; Sublet, Jack; Xiao, Limin; Chen, Jianhan; Roussel, Martine; Kriwacki, Richard. "Intrinsic disorder mediates the diverse regulatory functions of the Cdk inhibitor p21." Nat. Chem. Biol. 7, 214-221 (2011).
PubMed: 21358637

Assembly members:

Assembly members:
p21_kinase_inhibitory_domain, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p21_kinase_inhibitory_domain: RQNPCGSKACRRLFGPVDSE QLSRDCDALMAGCIQEARER WNFDFVTETPLEGDFAWERV RGLGLPKLYLPTGPRR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	92
15N chemical shifts	57
1H chemical shifts	57

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p21-KID	1

Entities:

Entity 1, p21-KID 76 residues - Formula weight is not available

1

ARG

GLN

ASN

PRO

CYS

GLY

SER

LYS

ALA

CYS

2

ARG

LEU

PHE

GLY

PRO

VAL

ASP

SER

GLU

3

GLN

LEU

SER

ARG

ASP

CYS

ASP

ALA

LEU

MET

4

ALA

GLY

CYS

ILE

GLN

GLU

ALA

ARG

GLU

ARG

5

TRP

ASN

PHE

ASP

PHE

VAL

THR

GLU

THR

PRO

6

LEU

GLU

GLY

ASP

PHE

ALA

TRP

GLU

ARG

VAL

7

ARG

GLY

LEU

GLY

LEU

PRO

LYS

LEU

TYR

LEU

8

PRO

THR

GLY

PRO

ARG

Samples:

sample_1: p21 kinase inhibitory domain, [U-100% 13C; U-100% 15N; U-80% 2H], 0.3 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D TROSY-HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

Bruker AMX 800 MHz

DBJ	BAD93118 BAE88691 BAG10980 BAG61011 BAG70104
EMBL	CAG38770 CAL37589 CAL37798
GB	AAA16109 AAA19811 AAA85641 AAB29246 AAB59559
PRF	2002363A
REF	NP_000380 NP_001181651 NP_001207706 NP_001207707 NP_001278478
SP	P38936
AlphaFold	P38936