BMRB Entry 17420

Name: Solution structure of Chr148 from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target Chr148
Published: 2011-02-04

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PDB ID: 2l8o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17420
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Chr148 from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target Chr148

Deposition date:

2011-01-21

Original release date:

2011-02-04

Authors:

Liu, Yizhou; Lee, Dan; Ciccosanti, Colleen; Nair, Lei MaoR; Rost, B.; Acton, T.B.; Xiao, R.; Everett, J.K; Montelione, G.T; Prestegard, James

Citation:

Citation: Liu, Yizhou; Lee, Dan; Ciccosanti, Colleen; Nair, Lei MaoR; Rost, B; Acton, T.B.; Xiao, R.; Everett, J.K; Montelione, G.T; Prestegard, James. "Solution structure of Chr148 from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target Chr148" .

Assembly members:

Assembly members:
Chr148, polymer, 144 residues, 16413.268 Da.

Natural source:

Natural source: Common Name: CFb group bacteria Taxonomy ID: 985 Superkingdom: Bacteria Kingdom: not available Genus/species: Cytophaga hutchinsonii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chr148: GNKITVEVTVYAAIEKVWKY WNEPAHIMKWCQASPEWHVP AAQNDLKAGGTFTTTMAAKD GSMSFDFGGVYDQVKTNDLI EYTIGDGRKVRIVFTHTGDT TNIVESFDPEETNPRELQQS GWQAILNSFKSYTENNLEHH HHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	548
15N chemical shifts	140
1H chemical shifts	768

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Chr148	1

Entities:

Entity 1, Chr148 144 residues - 16413.268 Da.

1

GLY

ASN

LYS

ILE

THR

VAL

GLU

VAL

THR

VAL

2

TYR

ALA

ILE

GLU

LYS

VAL

TRP

LYS

TYR

3

TRP

ASN

GLU

PRO

ALA

HIS

ILE

MET

LYS

TRP

4

CYS

GLN

ALA

SER

PRO

GLU

TRP

HIS

VAL

PRO

5

ALA

GLN

ASN

ASP

LEU

LYS

ALA

GLY

6

THR

PHE

THR

MET

ALA

LYS

ASP

7

GLY

SER

MET

SER

PHE

ASP

PHE

GLY

VAL

8

TYR

ASP

GLN

VAL

LYS

THR

ASN

ASP

LEU

ILE

9

GLU

TYR

THR

ILE

GLY

ASP

GLY

ARG

LYS

VAL

10

ARG

ILE

VAL

PHE

THR

HIS

THR

GLY

ASP

THR

11

THR

ASN

ILE

VAL

GLU

SER

PHE

ASP

PRO

GLU

12

GLU

THR

ASN

PRO

ARG

GLU

LEU

GLN

SER

13

GLY

TRP

GLN

ALA

ILE

LEU

ASN

SER

PHE

LYS

14

SER

TYR

THR

GLU

ASN

LEU

GLU

HIS

15

HIS

Samples:

sample_1: Chr148 1 mM; sodium azide 3 mM; DTT 10 mM; calcium chloride 5 mM; sodium chloride 200 mM; MES 20 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.3 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
NH-Jmod	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

Varian INOVA 600 MHz

PDB	2L8O
GB	ABG61021
REF	WP_011587126