BMRB Entry 17417

Name: mutant C335A, K309C of the Nek2 kinase leucine zipper
Published: 2011-04-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17417

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

mutant C335A, K309C of the Nek2 kinase leucine zipper

Deposition date:

2011-01-20

Original release date:

2011-04-08

Authors:

Pfuhl, Mark

Citation:

Citation: Croasdale, Rebecca; Ivins, Frank; Muskett, Fred; Daviter, Tina; Scott, David; Hardy, Tara; Smerdon, Steven; Fry, Andrew; Pfuhl, Mark. "An undecided coiled coil: the leucine zipper of Nek2 kinase exhibits atypical conformational exchange dynamics." J. Biol. Chem. 286, 27537-27547 (2011).
PubMed: 21669869

Assembly members:

Assembly members:
LZ5C335AK309C, polymer, 46 residues, 5438.1991 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LZ5C335AK309C: GAMAVLSELKLCEIQLQERE RALKAREERLEQKEQELAVR ERLAED

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	88
15N chemical shifts	46
1H chemical shifts	186

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LZ5, chain 1	1
2	LZ5, chain 2	1

Entities:

Entity 1, LZ5, chain 1 46 residues - 5438.1991 Da.

residues 1-4 are a cloning artifact

1

GLY

ALA

MET

ALA

VAL

LEU

SER

GLU

LEU

LYS

2

LEU

CYS

GLU

ILE

GLN

LEU

GLN

GLU

ARG

GLU

3

ARG

ALA

LEU

LYS

ALA

ARG

GLU

ARG

LEU

4

GLU

GLN

LYS

GLU

GLN

GLU

LEU

ALA

VAL

ARG

5

GLU

ARG

LEU

ALA

GLU

ASP

Samples:

sample_1: LZ5C335AK309C, [U-15N], 0.8 ± 0.1 mM; H2O 55 ± 0 M; sodium phosphate 20 ± 0.5 mM; sodium chloride 50 ± 0.5 mM; sodium azide 0.02 ± 0.0002 %; H2O 95%; D2O 5%

sample_2: LZ5C335AK309C, [U-13C; U-15N], 0.8 ± 0.1 mM; H2O 55 ± 0 M; sodium phosphate 20 ± 0.5 mM; sodium chloride 50 ± 0.5 mM; sodium azide 0.02 ± 0.0002 %; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 80 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 80 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_2
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_2

Software:

ANALYSIS v2.1, CCPN - chemical shift assignment, chemical shift calculation, peak picking

DANGLE v1.1, CCPN - calculate backbone torsion angles from chemical shifts

TOPSPIN v2.1, Bruker Biospin - processing

NMR spectrometers:

Bruker Avance 500 MHz