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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17412
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Rozenknop, Alexis; Rogov, Vladimir; Rogova, Natalia Yu; Lohr, Frank; Guntert, Peter; Dikic, Ivan; Dotsch, Volker. "Characterization of the Interaction of GABARAPL-1 with the LIR Motif of NBR1." J. Mol. Biol. 410, 477-487 (2011).
PubMed: 21620860
Assembly members:
GABARAPL-1, polymer, 119 residues, 14268.327 Da.
NBR1-LIR, polymer, 18 residues, 1840.027 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T1
Entity Sequences (FASTA):
GABARAPL-1: GSPEFKFQYKEDHPFEYRKK
EGEKIRKKYPDRVPVIVEKA
PKARVPDLDKRKYLVPSDLT
VGQFYFLIRKRIHLRPEDAL
FFFVNNTIPPTSATMGQLYE
DNHEEDYFLYVAYSDESVY
NBR1-LIR: GAMGSASSEDYIIILPES
Data type | Count |
13C chemical shifts | 506 |
15N chemical shifts | 110 |
1H chemical shifts | 881 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | GABARAPL-1 | 1 |
2 | NBR1-LIR | 2 |
Entity 1, GABARAPL-1 119 residues - 14268.327 Da.
Residues 1-5 represent a non-native cloning artefact
1 | GLY | SER | PRO | GLU | PHE | LYS | PHE | GLN | TYR | LYS | ||||
2 | GLU | ASP | HIS | PRO | PHE | GLU | TYR | ARG | LYS | LYS | ||||
3 | GLU | GLY | GLU | LYS | ILE | ARG | LYS | LYS | TYR | PRO | ||||
4 | ASP | ARG | VAL | PRO | VAL | ILE | VAL | GLU | LYS | ALA | ||||
5 | PRO | LYS | ALA | ARG | VAL | PRO | ASP | LEU | ASP | LYS | ||||
6 | ARG | LYS | TYR | LEU | VAL | PRO | SER | ASP | LEU | THR | ||||
7 | VAL | GLY | GLN | PHE | TYR | PHE | LEU | ILE | ARG | LYS | ||||
8 | ARG | ILE | HIS | LEU | ARG | PRO | GLU | ASP | ALA | LEU | ||||
9 | PHE | PHE | PHE | VAL | ASN | ASN | THR | ILE | PRO | PRO | ||||
10 | THR | SER | ALA | THR | MET | GLY | GLN | LEU | TYR | GLU | ||||
11 | ASP | ASN | HIS | GLU | GLU | ASP | TYR | PHE | LEU | TYR | ||||
12 | VAL | ALA | TYR | SER | ASP | GLU | SER | VAL | TYR |
Entity 2, NBR1-LIR 18 residues - 1840.027 Da.
Residues 1-4 and 18 represent a non-native cloning artefact
1 | GLY | ALA | MET | GLY | SER | ALA | SER | SER | GLU | ASP | ||||
2 | TYR | ILE | ILE | ILE | LEU | PRO | GLU | SER |
sample_1: GABARAPL-1, [U-98% 15N], 0.6 ± 0.05 mM; NBR1-LIR 0.9 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4.6 mM; protease inhibitor cocktail 1 mM; DSS 0.3 mM; H2O 95%; D2O 5%
sample_2: GABARAPL-1, [U-98% 13C; U-98% 15N], 0.6 ± 0.05 mM; NBR1-LIR 0.9 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4.6 mM; protease inhibitor cocktail 1 mM; DSS 0.3 mM; H2O 95%; D2O 5%
sample_3: GABARAPL-1 0.9 ± 0.05 mM; NBR1-LIR, [U-98% 15N], 0.6 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4.6 mM; protease inhibitor cocktail 1 mM; DSS 0.3 mM; H2O 95%; D2O 5%
sample_4: GABARAPL-1 0.9 ± 0.05 mM; NBR1-LIR, [U-98% 13C; U-98% 15N], 0.6 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 4.6 mM; protease inhibitor cocktail 1 mM; DSS 0.3 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_4 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_4 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_4 | isotropic | sample_conditions_1 |
3D HNCO | sample_2 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
3D HNCA | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
TROSY-H(CCCO)NH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
(H)CC(CO)NH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
3D HNCA | sample_4 | isotropic | sample_conditions_1 |
3D HNCACB | sample_4 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_4 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_4 | isotropic | sample_conditions_1 |
3D H(C)CH-TOCSY | sample_4 | isotropic | sample_conditions_1 |
TROSY-H(CCCO)NH-TOCSY | sample_4 | isotropic | sample_conditions_1 |
(H)CC(CO)NH-TOCSY | sample_4 | isotropic | sample_conditions_1 |
TOPSPIN, Bruker Biospin - collection, processing
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
TALOS, Cornilescu, Delaglio and Bax - structure solution
CSI, Wishart, D. S. & Sykes, B. D. - structure solution
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution
OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement
PDB | |
DBJ | BAA95100 BAB27950 BAB29146 BAB29690 BAB31345 |
EMBL | CAB66611 CAG38511 CAH89636 CAL38063 CAL38067 |
GB | AAH04602 AAH09309 AAH24706 AAH28315 AAH72921 |
REF | NP_001025652 NP_001028788 NP_001037759 NP_001085553 NP_001088067 |
SP | P60518 Q0VGK0 Q5BIZ2 Q5RF21 Q6GQ27 |
TPG | DAA29267 |
AlphaFold | Q5BIZ2 Q6GQ27 Q5RF21 Q0VGK0 P60518 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks