BMRB Entry 17383

Name: Crimean Congo Hemorrhagic Fever Gn zinc finger
Published: 2011-05-03

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PDB ID: 2l7x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17383
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Crimean Congo Hemorrhagic Fever Gn zinc finger

Deposition date:

2010-12-27

Original release date:

2011-05-03

Authors:

Estrada, David; De Guzman, Roberto

Citation:

Citation: Estrada, D. Fernando; De Guzman, Roberto. "Structural characterization of the crimean-congo hemorrhagic Fever virus gn tail provides insight into virus assembly." J. Biol. Chem. 286, 21678-21686 (2011).
PubMed: 21507948

Assembly members:

Assembly members:
CRIMEAN_CONGO_GN_ZINC_FINGER, polymer, 77 residues, 8936.340 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Crimean-Congo hemorrhagic fever virus Taxonomy ID: 11593 Superkingdom: viruses Kingdom: not available Genus/species: nairovirus Crimean-Congo hemorrhagic fever virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CRIMEAN_CONGO_GN_ZINC_FINGER: RELKPQTCTICETTPVNAID AEMHDLNCSYNICPYCASRL TSDGLARHVTQCPKRKEKVE ETELYLNLERIPWVVRK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
heteronucl_NOEs
- heteronuclear_noe_list_1

Data type	Count
13C chemical shifts	148
15N chemical shifts	70
1H chemical shifts	154
heteronuclear NOE values	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail	1
2	ZINC ION 1	2
3	ZINC ION 2	2

Entities:

Entity 1, Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail 77 residues - 8936.340 Da.

1

ARG

GLU

LEU

LYS

PRO

GLN

THR

CYS

THR

ILE

2

CYS

GLU

THR

PRO

VAL

ASN

ALA

ILE

ASP

3

ALA

GLU

MET

HIS

ASP

LEU

ASN

CYS

SER

TYR

4

ASN

ILE

CYS

PRO

TYR

CYS

ALA

SER

ARG

LEU

5

THR

SER

ASP

GLY

LEU

ALA

ARG

HIS

VAL

THR

6

GLN

CYS

PRO

LYS

ARG

LYS

GLU

LYS

VAL

GLU

7

GLU

THR

GLU

LEU

TYR

LEU

ASN

LEU

GLU

ARG

8

ILE

PRO

TRP

VAL

ARG

LYS

Entity 2, ZINC ION 1 - Zn - 65.409 Da.

1

ZN

Samples:

cchfv_gn_729-805_n15: CRIMEAN CONGO GN ZINC FINGER, [U-99% 15N], .7 mM; sodium phosphate 20 mM; sodium chloride 20 mM; zinc sulfate 0.1 mM; H2O 90%; D2O 10%

cchfv_gn_729-805_n15_c13: CRIMEAN CONGO GN ZINC FINGER, [U-99% 13C; U-99% 15N], .5 mM; sodium phosphate 20 mM; sodium chloride 20 mM; zinc sulfate 0.1 mM; H2O 90%; D2O 10%

cchfv_gn_729-805_n15_c13_D2O: CRIMEAN CONGO GN ZINC FINGER, [U-99% 13C; U-99% 15N], .7 mM; sodium phosphate 20 mM; sodium chloride 20 mM; zinc sulfate 0.1 mM; D2O 100%

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	cchfv_gn_729-805_n15	isotropic	sample_conditions_1
3D 1H-15N NOESY	cchfv_gn_729-805_n15	isotropic	sample_conditions_1
3D HNCA	cchfv_gn_729-805_n15_c13	isotropic	sample_conditions_1
3D HNCACB	cchfv_gn_729-805_n15_c13	isotropic	sample_conditions_1
3D HBHA(CO)NH	cchfv_gn_729-805_n15_c13	isotropic	sample_conditions_1
3D CBCA(CO)NH	cchfv_gn_729-805_n15_c13	isotropic	sample_conditions_1
3D 1H-13C NOESY	cchfv_gn_729-805_n15_c13_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	cchfv_gn_729-805_n15_c13_D2O	isotropic	sample_conditions_1
2D 1H-15N HeteroNuclear NOE	cchfv_gn_729-805_n15	isotropic	sample_conditions_1

Software:

CYANA, G ntert P. - refinement

AMBER v7, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2L7X
DBJ	BAB84574 BAB84575 BAB84577 BAB84578
EMBL	CAD61343 CAD61345
GB	AAA86616 AAK18286 AAM48106 AAM48107 AAP29978
REF	NP_950235
SP	Q8JSZ3
AlphaFold	Q8JSZ3