BMRB Entry 17382

Name: Solution structure of the human Raf-1 kinase inhibitor protein
Published: 2011-02-15

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PDB ID: 2l7w
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17382
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the human Raf-1 kinase inhibitor protein

Deposition date:

2011-01-27

Original release date:

2011-02-15

Authors:

Yi, C.

Citation:

Citation: Yi, Cuiying; Peng, Yu; Guo, Chenyun; Lin, Donghai. "1H, 13C, 15N backbone and side-chain resonance assignments of the human Raf-1 kinase inhibitor protein." Biomol. NMR Assignments 5, 63-66 (2011).
PubMed: 20924725

Assembly members:

Assembly members:
Raf-1_kinase_inhibitor_protein, polymer, 187 residues, 21086.967 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pReceiver-B01a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Raf-1_kinase_inhibitor_protein: MPVDLSKWSGPLSLQEVDEQ PQHPLHVTYAGAAVDELGKV LTPTQVKNRPTSISWDGLDS GKLYTLVLTDPDAPSRKDPK YREWHHFLVVNMKGNDISSG TVLSDYVGSGPPKGTGLHRY VWLVYEQDRPLKCDEPILSN RSGDHRGKFKVASFRKKYEL RAPVAGTCYQAEWDDYVPKL YEQLSGK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	755
15N chemical shifts	181
1H chemical shifts	1241

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Raf-1 kinase inhibitor protein	1

Entities:

Entity 1, Raf-1 kinase inhibitor protein 187 residues - 21086.967 Da.

1

MET

PRO

VAL

ASP

LEU

SER

LYS

TRP

SER

GLY

2

PRO

LEU

SER

LEU

GLN

GLU

VAL

ASP

GLU

GLN

3

PRO

GLN

HIS

PRO

LEU

HIS

VAL

THR

TYR

ALA

4

GLY

ALA

VAL

ASP

GLU

LEU

GLY

LYS

VAL

5

LEU

THR

PRO

THR

GLN

VAL

LYS

ASN

ARG

PRO

6

THR

SER

ILE

SER

TRP

ASP

GLY

LEU

ASP

SER

7

GLY

LYS

LEU

TYR

THR

LEU

VAL

LEU

THR

ASP

8

PRO

ASP

ALA

PRO

SER

ARG

LYS

ASP

PRO

LYS

9

TYR

ARG

GLU

TRP

HIS

PHE

LEU

VAL

10

ASN

MET

LYS

GLY

ASN

ASP

ILE

SER

GLY

11

THR

VAL

LEU

SER

ASP

TYR

VAL

GLY

SER

GLY

12

PRO

LYS

GLY

THR

GLY

LEU

HIS

ARG

TYR

13

VAL

TRP

LEU

VAL

TYR

GLU

GLN

ASP

ARG

PRO

14

LEU

LYS

CYS

ASP

GLU

PRO

ILE

LEU

SER

ASN

15

ARG

SER

GLY

ASP

HIS

ARG

GLY

LYS

PHE

LYS

16

VAL

ALA

SER

PHE

ARG

LYS

TYR

GLU

LEU

17

ARG

ALA

PRO

VAL

ALA

GLY

THR

CYS

TYR

GLN

18

ALA

GLU

TRP

ASP

TYR

VAL

PRO

LYS

LEU

19

TYR

GLU

GLN

LEU

SER

GLY

LYS

Samples:

sample_1: Raf-1 kinase inhibitor protein, [U-100% 13C; U-100% 15N], 3 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 200 mM; pH: 4.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 600 MHz

BMRB	16992 18204
PDB	1BD9 1BEH 2L7W 2QYQ
DBJ	BAA03684 BAE88027 BAE88359 BAG34868 BAG61396
EMBL	CAA51652 CAA53031 CAA59404 CAH93256
GB	AAB32876 AAD14234 AAH08714 AAH17396 AAH31102
PRF	2117380B
REF	NP_001126915 NP_001233128 NP_002558 XP_002753109 XP_004054017
SP	P30086 P48737 Q5R4R0
AlphaFold	P30086 P48737 Q5R4R0