BMRB Entry 17376

Name: Backbone Assignment of Human Proliferating Cell Nuclear Antigen Complexed with a 12 Amino Acids Peptide from the C-terminal Region of p21 (WAF1/CIP1)
Published: 2012-11-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17376

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Assignment of Human Proliferating Cell Nuclear Antigen Complexed with a 12 Amino Acids Peptide from the C-terminal Region of p21 (WAF1/CIP1)

Deposition date:

2010-12-22

Original release date:

2012-11-26

Authors:

Lopez-Alonso, Jorge; Sanchez, Ricardo; Blanco, Francisco; Campos-Olivas, Ramon

Citation:

Citation: De Biasio, Alfredo; Campos-Olivas, Ramon; Sanchez, Ricardo; Lopez-Alonso, Jorge; Pantoja-Uceda, David; Merino, Nekane; Villate, Maider; Martin-Garcia, Jose; Castillo, Francisco; Luque, Irene; Blanco, Francisco. "Proliferating Cell Nuclear Antigen (PCNA) Interactions in Solution Studied by NMR" PLoS One 7, e48390-e48390 (2012).
PubMed: 23139781

Assembly members:

Assembly members:
hPCNA, polymer, 264 residues, Formula weight is not available
p2112, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Rosetta BL21 (DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hPCNA: GPHMFEARLVQGSILKKVLE ALKDLINEACWDISSSGVNL QSMDSSHVSLVQLTLRSEGF DTYRCDRNLAMGVNLTSMSK ILKCAGNEDIITLRAEDNAD TLALVFEAPNQEKVSDYEMK LMDLDVEQLGIPEQEYSCVV KMPSGEFARICRDLSHIGDA VVISCAKDGVKFSASGELGN GNIKLSQTSNVDKEEEAVTI EMNEPVQLTFALRYLNFFTK ATPLSSTVTLSMSADVPLVV EYKIADMGHLKYYLAPKIED EEGS
p2112: KRRQTSMTDFYH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	676
15N chemical shifts	258
1H chemical shifts	276

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hPCNA monomer 1	1
2	hPCNA monomer 2	1
3	hPCNA monomer 3	1
4	P2112 molecule 1	2
5	P2112 molecule 2	2
6	P2112 molecule 3	2

Entities:

Entity 1, hPCNA monomer 1 264 residues - Formula weight is not available

Residues -3 to -1 are a non-native tag. The native sequence starts at residue number 4: MF...

1

GLY

PRO

HIS

MET

PHE

GLU

ALA

ARG

LEU

VAL

2

GLN

GLY

SER

ILE

LEU

LYS

VAL

LEU

GLU

3

ALA

LEU

LYS

ASP

LEU

ILE

ASN

GLU

ALA

CYS

4

TRP

ASP

ILE

SER

GLY

VAL

ASN

LEU

5

GLN

SER

MET

ASP

SER

HIS

VAL

SER

LEU

6

VAL

GLN

LEU

THR

LEU

ARG

SER

GLU

GLY

PHE

7

ASP

THR

TYR

ARG

CYS

ASP

ARG

ASN

LEU

ALA

8

MET

GLY

VAL

ASN

LEU

THR

SER

MET

SER

LYS

9

ILE

LEU

LYS

CYS

ALA

GLY

ASN

GLU

ASP

ILE

10

ILE

THR

LEU

ARG

ALA

GLU

ASP

ASN

ALA

ASP

11

THR

LEU

ALA

LEU

VAL

PHE

GLU

ALA

PRO

ASN

12

GLN

GLU

LYS

VAL

SER

ASP

TYR

GLU

MET

LYS

13

LEU

MET

ASP

LEU

ASP

VAL

GLU

GLN

LEU

GLY

14

ILE

PRO

GLU

GLN

GLU

TYR

SER

CYS

VAL

15

LYS

MET

PRO

SER

GLY

GLU

PHE

ALA

ARG

ILE

16

CYS

ARG

ASP

LEU

SER

HIS

ILE

GLY

ASP

ALA

17

VAL

ILE

SER

CYS

ALA

LYS

ASP

GLY

VAL

18

LYS

PHE

SER

ALA

SER

GLY

GLU

LEU

GLY

ASN

19

GLY

ASN

ILE

LYS

LEU

SER

GLN

THR

SER

ASN

20

VAL

ASP

LYS

GLU

ALA

VAL

THR

ILE

21

GLU

MET

ASN

GLU

PRO

VAL

GLN

LEU

THR

PHE

22

ALA

LEU

ARG

TYR

LEU

ASN

PHE

THR

LYS

23

ALA

THR

PRO

LEU

SER

THR

VAL

THR

LEU

24

SER

MET

SER

ALA

ASP

VAL

PRO

LEU

VAL

25

GLU

TYR

LYS

ILE

ALA

ASP

MET

GLY

HIS

LEU

26

LYS

TYR

LEU

ALA

PRO

LYS

ILE

GLU

ASP

27

GLU

GLY

SER

Entity 2, P2112 molecule 1 12 residues - Formula weight is not available

1

LYS

ARG

GLN

THR

SER

MET

THR

ASP

PHE

2

TYR

HIS

Samples:

sample_1: hPCNA, [U-13C; U-15N], 1.0 mM; p2112 1.5 mM; sodium chloride 137 mM; sodium phosphate 2.7 mM; potassium chloride 10 mM; potassium phosphate 2 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.327 M; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D trHNCACB	sample_1	isotropic	sample_conditions_1
3D trHNCO	sample_1	isotropic	sample_conditions_1
4D 1H-15N,1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz

BMRB	15501 17375
PDB	1AXC 1U76 1U7B 1UL1 1VYJ 1VYM 1W60 2ZVK 2ZVL 2ZVM 3P87 3TBL 3VKX 3WGW 4D2G 4RJF
DBJ	BAB28355 BAB28557 BAC40240 BAE30230 BAE30376
EMBL	CAA40938 CAA68261 CAG38740 CAG46598
GB	AAA35736 AAA60040 AAG10077 AAG37435 AAH00491
REF	NP_001029666 NP_001233697 NP_001253019 NP_001274649 NP_001278854
SP	P04961 P12004 P17918 P57761 P61258
TPG	DAA23435
AlphaFold	P04961 P12004 P17918 P57761 P61258