BMRB Entry 17362

Name: Backbone assignments for E2-25K
Published: 2010-12-16

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17362

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments for E2-25K

Deposition date:

2010-12-14

Original release date:

2010-12-16

Authors:

Wilson, Randall; Edmondson, Stephen; Twigg, Pamela

Citation:

Citation: Wilson, Randall; Edmondson, Stephen; Flatt, Justin; Helms, Kimberli; Twigg, Pamela. "The E2-25K ubiquitin-associated (UBA) domain aids in polyubiquitin chain synthesis and linkage specificity." Biochem. Biophys. Res. Commun. 405, 662-666 (2011).
PubMed: 21281599

Assembly members:

Assembly members:
E2-25K, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E2-25K: AMADIGSMANIAVQRIKREF KEVLKSEETSKNQIKVDLVD ENFTELRGEIAGPPDTPYEG GRYQLEIKIPETYPFNPPKV RFITKIWHPNISSVTGAICL DILKDQWAAAMTLRTVLLSL QALLAAAEPDDPQDAVVANQ YKQNPEMFKQTARLWAHVYA GAPVSSPEYTKKIENLCAMG FDRNAVIVALSSKSWDVETA TELLLSN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	558
15N chemical shifts	171
1H chemical shifts	171

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E2-25K monomer	1

Entities:

Entity 1, E2-25K monomer 207 residues - Formula weight is not available

Residues 1-7 represent the remainder of a non-native affinity tag

1

ALA

MET

ALA

ASP

ILE

GLY

SER

MET

ALA

ASN

2

ILE

ALA

VAL

GLN

ARG

ILE

LYS

ARG

GLU

PHE

3

LYS

GLU

VAL

LEU

LYS

SER

GLU

THR

SER

4

LYS

ASN

GLN

ILE

LYS

VAL

ASP

LEU

VAL

ASP

5

GLU

ASN

PHE

THR

GLU

LEU

ARG

GLY

GLU

ILE

6

ALA

GLY

PRO

ASP

THR

PRO

TYR

GLU

GLY

7

GLY

ARG

TYR

GLN

LEU

GLU

ILE

LYS

ILE

PRO

8

GLU

THR

TYR

PRO

PHE

ASN

PRO

LYS

VAL

9

ARG

PHE

ILE

THR

LYS

ILE

TRP

HIS

PRO

ASN

10

ILE

SER

VAL

THR

GLY

ALA

ILE

CYS

LEU

11

ASP

ILE

LEU

LYS

ASP

GLN

TRP

ALA

12

MET

THR

LEU

ARG

THR

VAL

LEU

SER

LEU

13

GLN

ALA

LEU

ALA

GLU

PRO

ASP

14

ASP

PRO

GLN

ASP

ALA

VAL

ALA

ASN

GLN

15

TYR

LYS

GLN

ASN

PRO

GLU

MET

PHE

LYS

GLN

16

THR

ALA

ARG

LEU

TRP

ALA

HIS

VAL

TYR

ALA

17

GLY

ALA

PRO

VAL

SER

PRO

GLU

TYR

THR

18

LYS

ILE

GLU

ASN

LEU

CYS

ALA

MET

GLY

19

PHE

ASP

ARG

ASN

ALA

VAL

ILE

VAL

ALA

LEU

20

SER

LYS

SER

TRP

ASP

VAL

GLU

THR

ALA

21

THR

GLU

LEU

SER

ASN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1

PDB	3E46 3F92 1YLA 2BEP 2BF8 2O25 3E46 3F92 3K9O 3K9P
DBJ	BAA24927 BAA78555 BAB24523 BAC29296 BAC33269
EMBL	CAG32430
GB	AAB19536 AAC50633 AAH02013 AAH22804 AAH41728
REF	NP_001005662 NP_001008611 NP_001026711 NP_001072139 NP_001080290
SP	P61085 P61086 P61087
TPG	DAA28715
AlphaFold	P61085 P61086 P61087

BMRB Entry 17362

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: