BMRB Entry 17353

Name: Androcam
Published: 2012-07-25

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PDB ID: 2lmt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17353
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Androcam

Deposition date:

2010-12-08

Original release date:

2012-07-25

Authors:

Joshi, Mehul; Moran, Sean; Kevin, MacKenzie

Citation:

Citation: Joshi, Mehul; Moran, Sean; MacKenzie, Kevin. "H, 15N and 13C chemical shifts of the D. melanogaster myosin VI light chain androcam in high calcium." Biomol. NMR Assignments 7, 171-174 (2013).
PubMed: 22706934

Assembly members:

Assembly members:
androcam, polymer, 148 residues, 17014.9 Da.
CA, non-polymer, Formula weight is not available

Natural source:

Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
androcam: MSELTEEQIAEFKDAFVQFD KEGTGKIATRELGTLMRTLG QNPTEAELQDLIAEAENNNN GQLNFTEFCGIMAKQMRETD TEEEMREAFKIFDRDGDGFI SPAELRFVMINLGEKVTDEE IDEMIREADFDGDGMINYEE FVWMISQK

Data sets:

assigned_chemical_shifts
- acam_shifts

Data type	Count
13C chemical shifts	662
15N chemical shifts	161
1H chemical shifts	1047

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	androcam	1
2	calcium ions	2

Entities:

Entity 1, androcam 148 residues - 17014.9 Da.

1

MET

SER

GLU

LEU

THR

GLU

GLN

ILE

ALA

2

GLU

PHE

LYS

ASP

ALA

PHE

VAL

GLN

PHE

ASP

3

LYS

GLU

GLY

THR

GLY

LYS

ILE

ALA

THR

ARG

4

GLU

LEU

GLY

THR

LEU

MET

ARG

THR

LEU

GLY

5

GLN

ASN

PRO

THR

GLU

ALA

GLU

LEU

GLN

ASP

6

LEU

ILE

ALA

GLU

ALA

GLU

ASN

7

GLY

GLN

LEU

ASN

PHE

THR

GLU

PHE

CYS

GLY

8

ILE

MET

ALA

LYS

GLN

MET

ARG

GLU

THR

ASP

9

THR

GLU

MET

ARG

GLU

ALA

PHE

LYS

10

ILE

PHE

ASP

ARG

ASP

GLY

ASP

GLY

PHE

ILE

11

SER

PRO

ALA

GLU

LEU

ARG

PHE

VAL

MET

ILE

12

ASN

LEU

GLY

GLU

LYS

VAL

THR

ASP

GLU

13

ILE

ASP

GLU

MET

ILE

ARG

GLU

ALA

ASP

PHE

14

ASP

GLY

ASP

GLY

MET

ILE

ASN

TYR

GLU

15

PHE

VAL

TRP

MET

ILE

SER

GLN

LYS

Entity 2, calcium ions - Formula weight is not available

1

CA

Samples:

sample_1: androcam, [U-100% 13C; U-100% 15N], 1.3 mM; Calcium chloride 10 uM; D2O 10%; H2O 90%; potassium chloride 10 mM; TRIS 10 mM; DTT 10 mM; potassium citrate 1 mM; TSP 0.1 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz

BMRB	17354
PDB	2LMT 2LMU 2LMV
EMBL	CAA53630
GB	AAF56511 AAL48405 ACL87451 ACL91891 ACZ95031
PRF	2021248D
REF	NP_001163737 NP_476988 XP_001981747 XP_002041300 XP_002098987
SP	P49258
AlphaFold	P49258