BMRB Entry 17331

Title:
NMR solution structure of GIP in Bicellular media   PubMed: 21539943
Deposition date:
2010-12-01
Original release date:
2011-06-01
Authors:
Venneti, Kalyana; Alana, Inigo; O'Harte, Finbarr; Malthouse, Paul; Hewage, Chandralal
Citation:

Citation: Venneti, Kalyana; Malthouse, J. Paul G; Hewage, Finbarr. "Conformational, receptor interaction and alanine scan studies of glucose-dependent insulinotropic polypeptide."  Biochim. Biophys. Acta 1814, 882-888 (2011).

Assembly members:

Assembly members:
entity, polymer, 42 residues, 4990.634 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: YAEGTFISDYSIAMDKIHQQ DFVNWLLAQKGKKNDWKHNI TQ

Data sets:
Data typeCount
1H chemical shifts294

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GIP in Bicellular media1

Entities:

Entity 1, GIP in Bicellular media 42 residues - 4990.634 Da.

1   TYRALAGLUGLYTHRPHEILESERASPTYR
2   SERILEALAMETASPLYSILEHISGLNGLN
3   ASPPHEVALASNTRPLEULEUALAGLNLYS
4   GLYLYSLYSASNASPTRPLYSHISASNILE
5   THRGLN

Samples:

sample_1: DHPC 100 mM; DMPC 250 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.9; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 800 MHz
  • Bruker DRX 900 MHz

Related Database Links:

BMRB 17330
PDB
GB AAA53192 AAA88043 AAH69100 AAH69663 AAH69686
REF NP_004114 XP_001091383 XP_001172671 XP_002806900 XP_003278797
SP P09681
AlphaFold P09681