BMRB Entry 17326
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17326
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Citation: Aeschbacher, Thomas; Schubert, Mario; Allain, Frederic H-T. "A procedure to validate and correct the (13)C chemical shift calibration of RNA datasets." J. Biomol. NMR 52, 179-190 (2012).
PubMed: 22252483
Assembly members:
RP1, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas fluorescens Taxonomy ID: 294 Superkingdom: bacteria Kingdom: not available Genus/species: Pseudomonas fluorescens
Experimental source: Production method: in vitro transcription Host organism: in vitro
Entity Sequences (FASTA):
RP1: GGGCUUCACGGAUGAAGCCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 54 |
| 1H chemical shifts | 60 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RP1 | 1 |
Entities:
Entity 1, RP1 20 residues - Formula weight is not available
nucleotides 1-4 and 17-20 are articifical G-C base pairs to stabilize the stem
| 1 | G | G | G | C | U | U | C | A | C | G | |
| 2 | G | A | U | G | A | A | G | C | C | C |
Samples:
sample_1: RP1 1.3 ± 0.1 mM; H2O 100%
sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.114, Goddard - chemical shift assignment
TOPSPIN v2.1, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 500 MHz