BMRB Entry 17316

Click here to enlarge.

PDB ID: 2kzl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17316
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Specifier domain and GA motif region of B. subtilis tyrS T box leader RNA

Deposition date:

2010-11-23

Original release date:

2011-04-12

Authors:

Wang, Jiachen

Citation:

Citation: Wang, Jiachen; Nikonowicz, Edward. "Solution Structure of the K-Turn and Specifier Loop Domains from the Bacillus subtilis tyrS T-Box Leader RNA." J. Mol. Biol. 408, 99-117 (2011).
PubMed: 21333656

Assembly members:

Assembly members:
Specifier_Domain_and_GA_motif_region_of_B._subtilis_tyrS_T_box_leader_RNA, polymer, 55 residues, 244.204 Da.

Natural source:

Natural source: Common Name: firmicutes Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: enzymatic semisynthesis Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Specifier_Domain_and_GA_motif_region_of_B._subtilis_tyrS_T_box_leader_RNA: GGGAGUAAAGAUUGAGACAA GUAGGACUUCGGUCCGAAUA CACUCAUGAACUCCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	347
15N chemical shifts	18
1H chemical shifts	408

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hairpin 1	1

Entities:

Entity 1, hairpin 1 55 residues - 244.204 Da.

1

G

A

G

U

A

G

2

A

U

G

A

G

A

C

A

3

G

U

A

G

A

C

U

C

4

G

U

C

G

A

U

A

5

C

A

C

U

C

A

U

G

A

6

C

U

C

Samples:

sample_1: Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA, [U-100% 13C; U-100% 15N], 1 mM; D2O 100%

sample_2: Specifier Domain and GA motif region of B. subtilis tyrS T box leader RNA, [U-100% 13C; U-100% 15N], 1 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	oriented	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCN	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C ct-spin-echo difference	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz