BMRB Entry 17313

Name: drosophila CstF-50 (1-92)
Published: 2011-01-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17313

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

drosophila CstF-50 (1-92)

Deposition date:

2010-11-22

Original release date:

2011-01-18

Authors:

Mackereth, Cameron

Citation:

Citation: Moreno-Morcillo, Maria; Minvielle-Sebastia, Lionel; Mackereth, Cameron; Fribourg, Sebastien. "Hexameric architecture of CstF supported by CstF-50 homodimerization domain structure." RNA 17, 412-418 (2011).
PubMed: 21233223

Assembly members:

Assembly members:
dCstF-50, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dCstF-50: MRDEILDPSNLVKNREILYR LMISQLMYDGLEKFAMELSM LVKADQCAPSERLLHVMIAG MQTLSDKDKTNSDDVLPGID LEFEPEASALAPGS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	259
15N chemical shifts	82
1H chemical shifts	82

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dCstF-50	1

Entities:

Entity 1, dCstF-50 94 residues - Formula weight is not available

C-terminal -GS arises from the cloning site in the vector Protein is expressed with an N-terminal His6-tag which is removed by TEV protease, leaving a GH- at the N-terminus

1

MET

ARG

ASP

GLU

ILE

LEU

ASP

PRO

SER

ASN

2

LEU

VAL

LYS

ASN

ARG

GLU

ILE

LEU

TYR

ARG

3

LEU

MET

ILE

SER

GLN

LEU

MET

TYR

ASP

GLY

4

LEU

GLU

LYS

PHE

ALA

MET

GLU

LEU

SER

MET

5

LEU

VAL

LYS

ALA

ASP

GLN

CYS

ALA

PRO

SER

6

GLU

ARG

LEU

HIS

VAL

MET

ILE

ALA

GLY

7

MET

GLN

THR

LEU

SER

ASP

LYS

ASP

LYS

THR

8

ASN

SER

ASP

VAL

LEU

PRO

GLY

ILE

ASP

9

LEU

GLU

PHE

GLU

PRO

GLU

ALA

SER

ALA

LEU

10

ALA

PRO

GLY

SER

Samples:

sample_1: dCstF-50, [U-99% 13C; U-99% 15N], 0.7 – 2 mM; KH2PO4 1.05 mM; Na2HPO4 3 mM; NaCl 154 mM; DTT 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 170 mM; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.0, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker Avance 700 MHz

BMRB	17308
PDB	2XZ2
GB	AAF57183 AAM11155 ACL84511 ACL89448 EDV52931
REF	NP_651883 XP_001981061 XP_002043730 XP_002105645