BMRB Entry 17301

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17301

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Title:
NMR Structure of an Archaeal DNA Binding Protein Sso7c4
Deposition date:
2010-11-16
Original release date:
2011-06-01
Authors:
Hsu, Chun-Hua; Wang, Andrew
Citation:

Citation: Hsu, Chun-Hua; Wang, Andrew H-J. "The DNA-recognition fold of Sso7c4 suggests a new member of SpoVT-AbrB superfamily from archaea."  Nucleic Acids Res. 39, 6764-6774 (2011).
PubMed: 21546550

Assembly members:

Assembly members:
Sso7c4, polymer, 53 residues, 6142 Da.

Natural source:

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sso7c4: AVEEIVKVSRNYQVTIPAKV RQKFQIKEGDLVKVTFDESE GVVKIQLLKEPWK

Data sets:
Data typeCount
15N chemical shifts55
1H chemical shifts415

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso7c4, Subunit 11
2Sso7c4, Subunit 21

Entities:

Entity 1, Sso7c4, Subunit 1 53 residues - 6142 Da.

1   ALAVALGLUGLUILEVALLYSVALSERARG
2   ASNTYRGLNVALTHRILEPROALALYSVAL
3   ARGGLNLYSPHEGLNILELYSGLUGLYASP
4   LEUVALLYSVALTHRPHEASPGLUSERGLU
5   GLYVALVALLYSILEGLNLEULEULYSGLU
6   PROTRPLYS

Samples:

sample_1: Sso7c4, [U-99% 15N], 1.5 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_2: Sso7c4 1 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 5.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.2, Goddard - chemical shift assignment

TOPSPIN v2.1, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB ACP36221 ACP38810 ACP46446 ACP47847 ACP56015
REF WP_012712036 WP_014511480 WP_029552626
SP P81552
AlphaFold P81552

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks