BMRB Entry 17299

Name: 1H, 13C and 15N Chemical shift assignments of the thioredoxin from the anaerobic bacteria Desulfovibrio vulgaris Hildenborough
Published: 2011-02-04

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PDB ID: 6zom
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17299
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical shift assignments of the thioredoxin from the anaerobic bacteria Desulfovibrio vulgaris Hildenborough

Deposition date:

2010-11-16

Original release date:

2011-02-04

Authors:

Garcin, Edwige; Bornet, Olivier; Sebban-Kreuzer, Corinne; Guerlesquin, Francoise

Citation:

Citation: Garcin, Edwige; Bornet, Olivier; Pieulle, Laetitia; Guerlesquin, Francoise; Sebban-Kreuzer, Corinne. "1H, 13C and 15N chemical shift assignments of the thioredoxin from the obligate anaerobe Desulfovibrio vulgaris Hildenborough." Biomol. NMR Assignments 5, 177-179 (2011).
PubMed: 21287302

Assembly members:

Assembly members:
Thioredoxin, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Desulfovibrio vulgaris Taxonomy ID: 881 Superkingdom: Bacteria Kingdom: not available Genus/species: Desulfovibrio vulgaris

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJF

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Thioredoxin: HHHHHHMAAQITDATFEASV LKSAIPVLIDFWAPWCGPCR AMGPVIDELAAEYEGKVLIV KMNVDDNPATPSKYGIRAIP TLILFKNGEVVEQVTGAVSK SSIKDMIAQKALG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	460
15N chemical shifts	110
1H chemical shifts	773

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	thioredoxin	1

Entities:

Entity 1, thioredoxin 113 residues - Formula weight is not available

1

HIS

MET

ALA

GLN

2

ILE

THR

ASP

ALA

THR

PHE

GLU

ALA

SER

VAL

3

LEU

LYS

SER

ALA

ILE

PRO

VAL

LEU

ILE

ASP

4

PHE

TRP

ALA

PRO

TRP

CYS

GLY

PRO

CYS

ARG

5

ALA

MET

GLY

PRO

VAL

ILE

ASP

GLU

LEU

ALA

6

ALA

GLU

TYR

GLU

GLY

LYS

VAL

LEU

ILE

VAL

7

LYS

MET

ASN

VAL

ASP

ASN

PRO

ALA

THR

8

PRO

SER

LYS

TYR

GLY

ILE

ARG

ALA

ILE

PRO

9

THR

LEU

ILE

LEU

PHE

LYS

ASN

GLY

GLU

VAL

10

VAL

GLU

GLN

VAL

THR

GLY

ALA

VAL

SER

LYS

11

SER

ILE

LYS

ASP

MET

ILE

ALA

GLN

LYS

12

ALA

LEU

GLY

Samples:

sample_1: oxidized thioredoxin, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O 10%; NaCl 100 mM; Potassium phosphate 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 5.5; pressure: 1 atm; temperature: 290 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	17300
GB	AAS96315 ABM28341 ADP86621
REF	WP_010939125 YP_011056