BMRB Entry 17290

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17290

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Title:
Backbone and side chain assignments for the intrinsically disordered cytoplasmic domain of human neuroligin-3
Deposition date:
2010-11-09
Original release date:
2011-05-02
Authors:
Wood, Kathleen; Paz, Aviv; Dijkstra, Klaas; Scheek, Ruud; Otten, Renee; Silman, Israel; Sussman, Joel; Mulder, Frans
Citation:

Citation: Wood, Kathleen; Paz, Aviv; Dijkstra, Klaas; Scheek, Ruud; Otten, Renee; Silman, Israel; Sussman, Joel; Mulder, Frans. "Backbone and side chain NMR assignments for the intrinsically disordered cytoplasmic domain of human neuroligin-3."  Biomol. NMR Assignments 6, 15-18 (2012).
PubMed: 21647611

Assembly members:

Assembly members:
hNLG3cyt, polymer, 139 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hNLG3cyt: MGSSHHHHHHSSGLVPRGSH MYRKDKRRQEPLRQPSPQRG AWAPELGAAPEEELAALQLG PTHHECEASPPHDTLRLTAL PDYTLTLRRSPDDIPLMTPN TITMIPNSLVGLQTLHPYNT FAAGFNSTGLPHSHSTTRV

Data sets:
Data typeCount
13C chemical shifts517
15N chemical shifts110
1H chemical shifts786

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hNLG3cyt1

Entities:

Entity 1, hNLG3cyt 139 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METTYRARGLYSASPLYSARGARGGLNGLU
4   PROLEUARGGLNPROSERPROGLNARGGLY
5   ALATRPALAPROGLULEUGLYALAALAPRO
6   GLUGLUGLULEUALAALALEUGLNLEUGLY
7   PROTHRHISHISGLUCYSGLUALASERPRO
8   PROHISASPTHRLEUARGLEUTHRALALEU
9   PROASPTYRTHRLEUTHRLEUARGARGSER
10   PROASPASPILEPROLEUMETTHRPROASN
11   THRILETHRMETILEPROASNSERLEUVAL
12   GLYLEUGLNTHRLEUHISPROTYRASNTHR
13   PHEALAALAGLYPHEASNSERTHRGLYLEU
14   PROHISSERHISSERTHRTHRARGVAL

Samples:

sample_1: hNLG3cyt, [U-99% 13C; U-99% 15N], 1 mM; DSS 0.1 mM; DTT 1 mM; azide 0.02%; potassium phosphate 20 mM; D2O 7%; H2O 93%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D C-TOCSY-(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
4D (HA)CACONHsample_1isotropicsample_conditions_1
3D (HN)CO(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESY-HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSY-HSQCsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D COCAHAsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HNcNsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

DBJ BAA96004 BAC11226 BAG10465 BAG37248 BAH12284
GB AAF71230 AAF71232 AAF71233 AAH51715 AAI23786
REF NP_001068972 NP_001126437 NP_001160132 NP_061850 NP_851820
SP Q9NZ94
TPG DAA12918
AlphaFold Q9NZ94

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks