BMRB Entry 17281
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17281
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Subasinghage, Anusha; Conlon, Donal; Hewage, J. Michael. "Conformational and membrane interaction studies of the antimicrobial peptide alyteserin-1c and its analogue [E4K]alyteserin-1c." Biochim. Biophys. Acta 1808, 1975-1984 (2011).
PubMed: 21565166
Assembly members:
Alyteserin-1C, polymer, 23 residues, 2270.737 Da.
Natural source: Common Name: midwife toad Taxonomy ID: 8443 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Alytes obstetricans
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Alyteserin-1C: GLKEIFKAGLGSLVKGIAAH
VAS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 140 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Alyteserin-1C | 1 |
Entities:
Entity 1, Alyteserin-1C 23 residues - 2270.737 Da.
| 1 | GLY | LEU | LYS | GLU | ILE | PHE | LYS | ALA | GLY | LEU | ||||
| 2 | GLY | SER | LEU | VAL | LYS | GLY | ILE | ALA | ALA | HIS | ||||
| 3 | VAL | ALA | SER |
Samples:
sample_1: DHPC 290 mM; H2O 90%; D2O 10%; Alyteserin-1C 1.54 mm
sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 307 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.0, Bruker Biospin - collection, data analysis, processing
SPARKY v3.114, Goddard - chemical shift assignment, peak picking
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution
SYBYL v6.8.1, Tripos - refinement
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB |