BMRB Entry 17272

Name: Solution structures of human PIWI-like 1 PAZ domain
Published: 2011-04-08

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PDB ID: 2l5c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17272
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of human PIWI-like 1 PAZ domain

Deposition date:

2010-11-01

Original release date:

2011-04-08

Authors:

Zeng, Lei; Zhang, Qiang; Yan, Kelley; Zhou, Ming-Ming

Citation:

Citation: Zeng, Lei; Zhang, Qiang; Yan, Kelley; Zhou, Ming-Ming. "Structural insights into piRNA recognition by the human PIWI-like 1 PAZ domain." Proteins 79, 2004-2009 (2011).
PubMed: 21465557

Assembly members:

Assembly members:
Human PIWI-like 1 PAZ domain, polymer, 134 residues, 15673.891 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX 4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human PIWI-like 1 PAZ domain: CTDVSHKVLRSETVLDFMFN FYHQTEEHKFQEQVSKELIG LVVLTKYNNKTYRVDDIDWD QNPKSTFKKADGSEVSFLEY YRKQYNQEITDLKQPVLVSQ PKRRRGPGGTLPGPAMLIPE LCYLTGLTDKMRND

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	456
15N chemical shifts	138
1H chemical shifts	878

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human PIWI-like 1 PAZ domain	1

Entities:

Entity 1, Human PIWI-like 1 PAZ domain 134 residues - 15673.891 Da.

1

CYS

THR

ASP

VAL

SER

HIS

LYS

VAL

LEU

ARG

2

SER

GLU

THR

VAL

LEU

ASP

PHE

MET

PHE

ASN

3

PHE

TYR

HIS

GLN

THR

GLU

HIS

LYS

PHE

4

GLN

GLU

GLN

VAL

SER

LYS

GLU

LEU

ILE

GLY

5

LEU

VAL

LEU

THR

LYS

TYR

ASN

LYS

6

THR

TYR

ARG

VAL

ASP

ILE

ASP

TRP

ASP

7

GLN

ASN

PRO

LYS

SER

THR

PHE

LYS

ALA

8

ASP

GLY

SER

GLU

VAL

SER

PHE

LEU

GLU

TYR

9

TYR

ARG

LYS

GLN

TYR

ASN

GLN

GLU

ILE

THR

10

ASP

LEU

LYS

GLN

PRO

VAL

LEU

VAL

SER

GLN

11

PRO

LYS

ARG

GLY

PRO

GLY

THR

12

LEU

PRO

GLY

PRO

ALA

MET

LEU

ILE

PRO

GLU

13

LEU

CYS

TYR

LEU

THR

GLY

LEU

THR

ASP

LYS

14

MET

ARG

ASN

ASP

Samples:

sample_1: sodium phosphate 100 mM; sodium chloride 150 mM; DTT, [U-100% 2H], 5 mM; D2O 100%

sample_2: sodium phosphate 100 mM; sodium chloride 150 mM; DTT, [U-100% 2H], 5 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 13C-Edited-13C/15N-filtered NOESY	sample_1	isotropic	sample_conditions_1

Software:

ARIA v2.2, Linge, O'Donoghue and Nilges - refinement

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift calculation, processing

NMRView v5.04, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz
Bruker DRX 500 MHz

BMRB	17273
PDB	2L5C 2L5D 2XFM 3O3I 3O6E 3O7V
DBJ	BAA93705 BAC04068 BAF49084
GB	AAC97371 AAH28581 AAH66846 AAI29858 AAI29859
REF	NP_001102323 NP_001177900 NP_001181902 NP_001182640 NP_001302505
SP	Q96J94 Q9JMB7
AlphaFold	Q96J94 Q9JMB7