BMRB Entry 17249

Title:
1H, 13C and 15N resonances of the AlgE62 subunit from Azotobacter vinelandii mannuronan C5-epimerase
Deposition date:
2010-10-12
Original release date:
2011-01-18
Authors:
Andressen, Trygve; Buchinger, Edith; Skjaak-Break, Gudmund; Valla, Svein; Aachmann, Finn
Citation:

Citation: Andreassen, Trygve; Buchinger, Edith; Skjak-Braek, Gudmund; Valla, Svein; Aachmann, Finn. "1H, 13C and 15N resonances of the AlgE62 subunit from Azotobacter vinelandii mannuronan C5-epimerase."  Biomol. NMR Assignments 5, 147-149 (2011).
PubMed: 21188559

Assembly members:

Assembly members:
AlgE6R2, polymer, 161 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Azotobacter vinelandii   Taxonomy ID: 354   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Azotobacter vinelandii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFA12

Data sets:
Data typeCount
13C chemical shifts606
15N chemical shifts160
1H chemical shifts941

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R21
2calcium ion2

Entities:

Entity 1, R2 161 residues - Formula weight is not available

1   ASPPROSERALAGLUALAGLNPROILEVAL
2   GLYSERASPLEUASPASPGLNLEUHISGLY
3   THRLEULEUGLYGLUGLUILESERGLYGLY
4   GLYGLYALAASPGLNLEUTYRGLYTYRGLY
5   GLYGLYASPLEULEUASPGLYGLYALAGLY
6   ARGASPARGLEUTHRGLYGLYGLUGLYALA
7   ASPTHRPHEARGPHEALALEUARGGLUASP
8   SERGLNARGSERALAALAGLYTHRPHESER
9   ASPLEUILELEUASPPHEASPPROTHRGLN
10   ASPLYSLEUASPVALSERALALEUGLYPHE
11   THRGLYLEUGLYASNGLYTYRALAGLYTHR
12   LEUALAVALSERVALSERASPASPGLYTHR
13   ARGTHRTYRLEULYSSERTYRGLUTHRASP
14   ALAGLUGLYARGSERPHEGLUVALSERLEU
15   GLNGLYASNHISALAALAALALEUSERALA
16   ASPASNILELEUPHEALATHRPROVALPRO
17   VAL

Entity 2, calcium ion - Ca - 40.078 Da.

1   CA

Samples:

AlgE6R2_H2O: AlgE6R2, [U-98% 13C; U-98% 15N], 1 – 1.2 mM; calcium chloride 25 ± 0.1 mM; HEPES 20 ± 0.1 mM; H2O 90%; D2O 10%

AlgE6R2_D2O: AlgE6R2, [U-98% 13C; U-98% 15N], 1 – 1.2 mM; calcium chloride 25 ± 0.1 mM; HEPES 20 ± 0.1 mM; D2O 100%

nonAlgE6R2_D2O: AlgE6R21 – 1.2 mM; calcium chloride 25 ± 0.1 mM; HEPES 20 ± 0.1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.165 M; pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCAlgE6R2_H2Oisotropicsample_conditions_1
2D 1H-13C HSQCAlgE6R2_D2Oisotropicsample_conditions_1
3D CBCA(CO)NHAlgE6R2_H2Oisotropicsample_conditions_1
3D HNCOAlgE6R2_H2Oisotropicsample_conditions_1
2D 1H-1H TOCSYnonAlgE6R2_D2Oisotropicsample_conditions_1
2D 1H-1H COSYnonAlgE6R2_D2Oisotropicsample_conditions_1
2D 1H-1H TOCSYnonAlgE6R2_D2Oisotropicsample_conditions_1
3D HNCAAlgE6R2_H2Oisotropicsample_conditions_1
3D HBHA(CO)NHAlgE6R2_H2Oisotropicsample_conditions_1
3D HN(CO)CAAlgE6R2_H2Oisotropicsample_conditions_1
3D HCCH-TOCSYAlgE6R2_D2Oisotropicsample_conditions_1
3D HCCH-COSYAlgE6R2_D2Oisotropicsample_conditions_1
3D HBHANHAlgE6R2_H2Oisotropicsample_conditions_1
3D CBCANHAlgE6R2_H2Oisotropicsample_conditions_1
3D HN(CA)COAlgE6R2_H2Oisotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

CARA v1.4.1; 1.5.2, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks