BMRB Entry 17245

Title:
Solution Structure of the Chemokine CCL21
Deposition date:
2010-10-10
Original release date:
2012-02-03
Authors:
Veldkamp, C.; Peterson, F.; Love, M.; Sandberg, J.
Citation:

Citation: Love, Melissa; Sandberg, Jamie; Ziarek, Joshua; Gerarden, Kyle; Rode, Renee; Jensen, Davin; McCaslin, Darrell; Peterson, Francis; Veldkamp, Christopher. "Solution Structure of CCL21 and Identification of a Putative CCR7 Binding Site."  Biochemistry 51, 733-735 (2012).
PubMed: 22221265

Assembly members:

Assembly members:
CCL21_SLC_6Ckine_Exodus-2, polymer, 113 residues, 12519.708 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30-8HT-SMT3

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts441
15N chemical shifts99
1H chemical shifts684

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CCL21_SLC_6Ckine_Exodus-21

Entities:

Entity 1, CCL21_SLC_6Ckine_Exodus-2 113 residues - 12519.708 Da.

1   SERASPGLYGLYALAGLNASPCYSCYSLEU
2   LYSTYRSERGLNARGLYSILEPROALALYS
3   VALVALARGSERTYRARGLYSGLNGLUPRO
4   SERLEUGLYCYSSERILEPROALAILELEU
5   PHELEUPROARGLYSARGSERGLNALAGLU
6   LEUCYSALAASPPROLYSGLULEUTRPVAL
7   GLNGLNLEUMETGLNHISLEUASPLYSTHR
8   PROSERPROGLNLYSPROALAGLNGLYCYS
9   ARGLYSASPARGGLYALASERLYSTHRGLY
10   LYSLYSGLYLYSGLYSERLYSGLYCYSLYS
11   ARGTHRGLUARGSERGLNTHRPROLYSGLY
12   PROLYSLEU

Samples:

sample_1: CCL21_SLC_6Ckine_Exodus-2, [U-100% 13C; U-100% 15N], 0.500 mM; D2O 10%; MES, [U-2H], 25 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 21 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

XEASY, Bartels et al. - chemical shift assignment, data analysis

GARANT, Bartels, Guntert, Billeter and Wuthrich - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAA21817 BAG34842 BAG72999
EMBL CAA06653 CAG29322
GB AAB86594 AAB91454 AAH27918 AAQ13417 AAQ89246
REF NP_002980 XP_001164162 XP_003263432 XP_003829862 XP_004048021
SP O00585
AlphaFold O00585

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks