BMRB Entry 17240

Name: INFLUENZA HEMEGGLUTININ FUSION PEPTIDE MUTANT G13A
Published: 2010-10-19

Click here to enlarge.

PDB ID: 2l4g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17240
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

INFLUENZA HEMEGGLUTININ FUSION PEPTIDE MUTANT G13A

Deposition date:

2010-10-05

Original release date:

2010-10-19

Authors:

Lai, Alex; Tamm, Lukas

Citation:

Citation: Lai, Alex; Tamm, Lukas. "Shallow Boomerang-shaped Influenza Hemagglutinin G13A Mutant Structure Promotes Leaky Membrane Fusion." J. Biol. Chem. 285, 37467-37475 (2010).
PubMed: 20826788

Assembly members:

Assembly members:
Influenza_HA_fusion_peptide_G13A, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Influenza A virus Taxonomy ID: 11320 Superkingdom: virus Kingdom: not available Genus/species: Influenzavirus A not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET31b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Influenza_HA_fusion_peptide_G13A: GLFGAIAGFIENAWEGMIDG GCGKKKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	117

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Influenza HA fusion peptide(G13A)	1

Entities:

Entity 1, Influenza HA fusion peptide(G13A) 27 residues - Formula weight is not available

1

GLY

LEU

PHE

GLY

ALA

ILE

ALA

GLY

PHE

ILE

2

GLU

ASN

ALA

TRP

GLU

GLY

MET

ILE

ASP

GLY

3

GLY

CYS

GLY

LYS

Samples:

FP_N15: Influenza HA fusion peptide G13A, [U-15N], 1 mM; DPC, [U-2H], 200 mM; acetic acid, [U-2H], 20 mM; DTT 5 mM; H2O 95%; D2O 5%

FP: Influenza HA fusion peptide G13A, [U-15N], 2 mM; DPC, [U-2H], 200 mM; acetic acid, [U-2H], 20 mM; DTT 5 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	FP	isotropic	sample_conditions_1
2D 1H-1H NOESY	FP	isotropic	sample_conditions_1
3D HNHA	FP_N15	isotropic	sample_conditions_1
3D 1H-15N NOESY	FP_N15	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Varian INOVA 600 MHz