BMRB Entry 17231

Name: Resonance assignments of ArsD
Published: 2010-11-15

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17231

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of ArsD

Deposition date:

2010-10-01

Original release date:

2010-11-15

Authors:

Ye, Jun; He, Yanan; Skalicky, Jack; Rosen, Barry; Stemmler, Timothy

Citation:

Citation: Ye, Jun; He, Yanan; Skalicky, Jack; Rosen, Barry; Stemmler, Timothy. "Resonance assignments and secondary structure prediction of the As(III) metallochaperone ArsD in solution." Biomol. NMR Assignments 5, 109-112 (2011).
PubMed: 21063813

Assembly members:

Assembly members:
ArsD, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a-ArsD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ArsD: MKTLMVFDPAMCCSTGVCGT DVDQALVDFSTDVQWLKQCG VQIERFNLAQQPMSFVQNEK VKAFIEASGAEGLPLLLLDG ETVMAGRYPKRAELARWFGI PLDKVGLAPSGCCGGNTSHH HHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	336
15N chemical shifts	109
1H chemical shifts	109

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ArsD_1	1
2	ArsD_2	1

Entities:

Entity 1, ArsD_1 124 residues - Formula weight is not available

1

MET

LYS

THR

LEU

MET

VAL

PHE

ASP

PRO

ALA

2

MET

CYS

SER

THR

GLY

VAL

CYS

GLY

THR

3

ASP

VAL

ASP

GLN

ALA

LEU

VAL

ASP

PHE

SER

4

THR

ASP

VAL

GLN

TRP

LEU

LYS

GLN

CYS

GLY

5

VAL

GLN

ILE

GLU

ARG

PHE

ASN

LEU

ALA

GLN

6

GLN

PRO

MET

SER

PHE

VAL

GLN

ASN

GLU

LYS

7

VAL

LYS

ALA

PHE

ILE

GLU

ALA

SER

GLY

ALA

8

GLU

GLY

LEU

PRO

LEU

ASP

GLY

9

GLU

THR

VAL

MET

ALA

GLY

ARG

TYR

PRO

LYS

10

ARG

ALA

GLU

LEU

ALA

ARG

TRP

PHE

GLY

ILE

11

PRO

LEU

ASP

LYS

VAL

GLY

LEU

ALA

PRO

SER

12

GLY

CYS

GLY

ASN

THR

SER

HIS

13

HIS

Samples:

sample_1: ArsD, [U-98% 13C; U-98% 15N], 1 mM; potassium phosphate 20 mM; sodium chloride 100 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.22 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 720 MHz

PDB	3KGK 3MWH
DBJ	BAB91570
EMBL	CSO49052
GB	AAA93060 AJE59284 KNW56142
REF	NP_863362 WP_011117596 WP_032488785 YP_009074720
SP	P46003
AlphaFold	P46003