BMRB Entry 17224
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17224
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Frickel, Eva-Maria; Riek, Roland; Jelesarov, Ilian; Helenius, Ari; Wuthrich, Kurt; Ellgaard, Lars. "TROSY-NMR reveals interaction between ERp57 and the tip of the calreticulin P-domain" Proc. Natl. Acad. Sci. U.S.A. 99, 1954-1959 (2002).
PubMed: 11842220
Assembly members:
CRT, polymer, 100 residues, Formula weight is not available
ERp57, polymer, 505 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metaoza Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHisERp57
Entity Sequences (FASTA):
CRT: SQVESGSLEDDWDFLPPKKI
KDPDASKPEDWDERAKIDDP
TDSKPEDWDKPEHIPDPDAK
KPEDWDEEMDGEWEPPVIQN
PEYKGEWKPRQIDNPDYKGT
ERp57: MRLRRLALFPGVALLLAAAR
LAAASDVLELTDDNFESRIS
DTGSAGLMLVEFFAPWCGHC
KRLAPEYEAAATRLKGIVPL
AKVDCTANTNTCNKYGVSGY
PTLKIFRDGEEAGAYDGPRT
ADGIVSHLKKQAGPASVPLR
TEEEFKKFISDKDASIVGFF
DDSFSEAHSEFLKAASNLRD
NYRFAHTNVESLVNEYDDNG
EGIILFRPSHLTNKFEDKTV
AYTEQKMTSGKIKKFIQENI
FGICPHMTEDNKDLIQGKDL
LIAYYDVDYEKNAKGSNYWR
NRVMMVAKKFLDAGHKLNFA
VASRKTFSHELSDFGLESTA
GEIPVVAIRTAKGEKFVMQE
EFSRDGKALERFLQDYFDGN
LKRYLKSEPIPESNDGPVKV
VVAENFDEIVNNENKDVLIE
FYAPWCGHCKNLEPKYKELG
EKLSKDPNIVIAKMDATAND
VPSPYEVRGFPTIYFSPANK
KLNPKKYEGGRELSDFISYL
QREATNPPVIQEEKPKKKKK
AQEDL
- binding_constants
- chemical_rates
| Data type | Count |
| binding constants | 7 |
| kinetic rates | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CRT | 1 |
| 2 | ERp57 | 2 |
Entities:
Entity 1, CRT 100 residues - Formula weight is not available
| 1 | SER | GLN | VAL | GLU | SER | GLY | SER | LEU | GLU | ASP | |
| 2 | ASP | TRP | ASP | PHE | LEU | PRO | PRO | LYS | LYS | ILE | |
| 3 | LYS | ASP | PRO | ASP | ALA | SER | LYS | PRO | GLU | ASP | |
| 4 | TRP | ASP | GLU | ARG | ALA | LYS | ILE | ASP | ASP | PRO | |
| 5 | THR | ASP | SER | LYS | PRO | GLU | ASP | TRP | ASP | LYS | |
| 6 | PRO | GLU | HIS | ILE | PRO | ASP | PRO | ASP | ALA | LYS | |
| 7 | LYS | PRO | GLU | ASP | TRP | ASP | GLU | GLU | MET | ASP | |
| 8 | GLY | GLU | TRP | GLU | PRO | PRO | VAL | ILE | GLN | ASN | |
| 9 | PRO | GLU | TYR | LYS | GLY | GLU | TRP | LYS | PRO | ARG | |
| 10 | GLN | ILE | ASP | ASN | PRO | ASP | TYR | LYS | GLY | THR |
Entity 2, ERp57 505 residues - Formula weight is not available
| 1 | MET | ARG | LEU | ARG | ARG | LEU | ALA | LEU | PHE | PRO | ||||
| 2 | GLY | VAL | ALA | LEU | LEU | LEU | ALA | ALA | ALA | ARG | ||||
| 3 | LEU | ALA | ALA | ALA | SER | ASP | VAL | LEU | GLU | LEU | ||||
| 4 | THR | ASP | ASP | ASN | PHE | GLU | SER | ARG | ILE | SER | ||||
| 5 | ASP | THR | GLY | SER | ALA | GLY | LEU | MET | LEU | VAL | ||||
| 6 | GLU | PHE | PHE | ALA | PRO | TRP | CYS | GLY | HIS | CYS | ||||
| 7 | LYS | ARG | LEU | ALA | PRO | GLU | TYR | GLU | ALA | ALA | ||||
| 8 | ALA | THR | ARG | LEU | LYS | GLY | ILE | VAL | PRO | LEU | ||||
| 9 | ALA | LYS | VAL | ASP | CYS | THR | ALA | ASN | THR | ASN | ||||
| 10 | THR | CYS | ASN | LYS | TYR | GLY | VAL | SER | GLY | TYR | ||||
| 11 | PRO | THR | LEU | LYS | ILE | PHE | ARG | ASP | GLY | GLU | ||||
| 12 | GLU | ALA | GLY | ALA | TYR | ASP | GLY | PRO | ARG | THR | ||||
| 13 | ALA | ASP | GLY | ILE | VAL | SER | HIS | LEU | LYS | LYS | ||||
| 14 | GLN | ALA | GLY | PRO | ALA | SER | VAL | PRO | LEU | ARG | ||||
| 15 | THR | GLU | GLU | GLU | PHE | LYS | LYS | PHE | ILE | SER | ||||
| 16 | ASP | LYS | ASP | ALA | SER | ILE | VAL | GLY | PHE | PHE | ||||
| 17 | ASP | ASP | SER | PHE | SER | GLU | ALA | HIS | SER | GLU | ||||
| 18 | PHE | LEU | LYS | ALA | ALA | SER | ASN | LEU | ARG | ASP | ||||
| 19 | ASN | TYR | ARG | PHE | ALA | HIS | THR | ASN | VAL | GLU | ||||
| 20 | SER | LEU | VAL | ASN | GLU | TYR | ASP | ASP | ASN | GLY | ||||
| 21 | GLU | GLY | ILE | ILE | LEU | PHE | ARG | PRO | SER | HIS | ||||
| 22 | LEU | THR | ASN | LYS | PHE | GLU | ASP | LYS | THR | VAL | ||||
| 23 | ALA | TYR | THR | GLU | GLN | LYS | MET | THR | SER | GLY | ||||
| 24 | LYS | ILE | LYS | LYS | PHE | ILE | GLN | GLU | ASN | ILE | ||||
| 25 | PHE | GLY | ILE | CYS | PRO | HIS | MET | THR | GLU | ASP | ||||
| 26 | ASN | LYS | ASP | LEU | ILE | GLN | GLY | LYS | ASP | LEU | ||||
| 27 | LEU | ILE | ALA | TYR | TYR | ASP | VAL | ASP | TYR | GLU | ||||
| 28 | LYS | ASN | ALA | LYS | GLY | SER | ASN | TYR | TRP | ARG | ||||
| 29 | ASN | ARG | VAL | MET | MET | VAL | ALA | LYS | LYS | PHE | ||||
| 30 | LEU | ASP | ALA | GLY | HIS | LYS | LEU | ASN | PHE | ALA | ||||
| 31 | VAL | ALA | SER | ARG | LYS | THR | PHE | SER | HIS | GLU | ||||
| 32 | LEU | SER | ASP | PHE | GLY | LEU | GLU | SER | THR | ALA | ||||
| 33 | GLY | GLU | ILE | PRO | VAL | VAL | ALA | ILE | ARG | THR | ||||
| 34 | ALA | LYS | GLY | GLU | LYS | PHE | VAL | MET | GLN | GLU | ||||
| 35 | GLU | PHE | SER | ARG | ASP | GLY | LYS | ALA | LEU | GLU | ||||
| 36 | ARG | PHE | LEU | GLN | ASP | TYR | PHE | ASP | GLY | ASN | ||||
| 37 | LEU | LYS | ARG | TYR | LEU | LYS | SER | GLU | PRO | ILE | ||||
| 38 | PRO | GLU | SER | ASN | ASP | GLY | PRO | VAL | LYS | VAL | ||||
| 39 | VAL | VAL | ALA | GLU | ASN | PHE | ASP | GLU | ILE | VAL | ||||
| 40 | ASN | ASN | GLU | ASN | LYS | ASP | VAL | LEU | ILE | GLU | ||||
| 41 | PHE | TYR | ALA | PRO | TRP | CYS | GLY | HIS | CYS | LYS | ||||
| 42 | ASN | LEU | GLU | PRO | LYS | TYR | LYS | GLU | LEU | GLY | ||||
| 43 | GLU | LYS | LEU | SER | LYS | ASP | PRO | ASN | ILE | VAL | ||||
| 44 | ILE | ALA | LYS | MET | ASP | ALA | THR | ALA | ASN | ASP | ||||
| 45 | VAL | PRO | SER | PRO | TYR | GLU | VAL | ARG | GLY | PHE | ||||
| 46 | PRO | THR | ILE | TYR | PHE | SER | PRO | ALA | ASN | LYS | ||||
| 47 | LYS | LEU | ASN | PRO | LYS | LYS | TYR | GLU | GLY | GLY | ||||
| 48 | ARG | GLU | LEU | SER | ASP | PHE | ILE | SER | TYR | LEU | ||||
| 49 | GLN | ARG | GLU | ALA | THR | ASN | PRO | PRO | VAL | ILE | ||||
| 50 | GLN | GLU | GLU | LYS | PRO | LYS | LYS | LYS | LYS | LYS | ||||
| 51 | ALA | GLN | GLU | ASP | LEU |
Samples:
sample_1: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.14 mM; ERp57 0.38 mM
sample_2: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.12 mM; ERp57 0.33 mM
sample_3: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.11 mM; ERp57 0.29 mM
sample_4: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.1 mM; ERp57 0.26 mM
sample_5: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.09 mM; ERp57 0.24 mM
sample_6: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.28 mM
sample_7: potassium dihydrogen phosphate 100 mM; sodium chloride 25 mM; beta-mercaptoethanol 10 mM; CRT, [U-15N, U2H], 0.14 mM; ERp57 0.07 mM
sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 1H-15N TROSY | sample_2 | isotropic | sample_conditions_1 |
| 1H-15N TROSY | sample_3 | isotropic | sample_conditions_1 |
| 1H-15N TROSY | sample_4 | isotropic | sample_conditions_1 |
| 1H-15N TROSY | sample_5 | isotropic | sample_conditions_1 |
| 1H-15N TROSY | sample_6 | isotropic | sample_conditions_1 |
| 1H-15N TROSY | sample_7 | isotropic | sample_conditions_1 |
Software:
PROSA, Gintert, P., Dotsch, V., Wider,G . & WiithrichK, . - processing
XEASY, Bartels et al. - data analysis
NMR spectrometers:
- Bruker DRX 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAA11345 BAC35852 BAD96780 BAE01267 BAE22866 BAA03759 BAA11928 BAI45467 BAK62544 |
| EMBL | CAA33053 CAA37446 CAA55890 CAG33351 CAA89996 CAH90193 |
| GB | AAA31188 AAA36582 AAA37569 AAA51916 AAB20096 AAB37397 AAC50331 AAC51518 AAH14433 AAH36000 |
| REF | NP_001075704 NP_001167604 NP_001231051 NP_001248060 NP_001274539 NP_001127250 NP_001162437 NP_001182645 NP_001233381 NP_005304 |
| SP | P14211 P15253 P18418 P27797 P28491 P30101 Q4VIT4 Q5RDG4 |
| PRF | 2201310A 2209333A |
| AlphaFold | P14211 P15253 P18418 P27797 P28491 P30101 Q4VIT4 Q5RDG4 |