BMRB Entry 17222

Name: lac repressor-lac operator interaction: NMR observations
Published: 2010-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17222

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

lac repressor-lac operator interaction: NMR observations

Deposition date:

2010-09-29

Original release date:

2010-11-10

Authors:

Nick, Harry; Arndt, Kim; Boschelli, Frank; Jarema, Mary Ann; Lillis, Marcella; Sadler, John; Caruthers, Marvin; Lu, Ponzy

Citation:

Citation: Nick, Harry; Arndt, Kim; Boschelli, Frank; Jarema, Mary Ann; Lillis, Marcella; Sadler, John; Caruthers, Marvin; Lu, Ponzy. "lac repressor-lac operator interaction: NMR observations" Proc. Natl. Acad. Sci. U.S.A. 79, 218-222 (1982).
PubMed: 7043455

Assembly members:

Assembly members:
operator, polymer, 73 residues, Formula weight is not available
repressor, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source: Common Name: cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pOE101

Entity Sequences (FASTA):

Entity Sequences (FASTA):
operator: CCACATGTGGAATTGTGAGC GGATAACAATTTGTGGGGTG TACACCTTAACACTCGCCTA TTGTTAAACACC
repressor: MXXXHYL

Data sets:

chemical rates

Data type	Count
binding constants	9
kinetic rates	1

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	operator	1
2	repressor	2

Entities:

Entity 1, operator 73 residues - Formula weight is not available

1

DC

DA

DC

DA

DT

DG

DT

DG

2

DA

DT

DG

DT

DG

DA

DG

DC

3

DG

DA

DT

DA

DC

DA

DT

4

DT

DG

DT

DG

DT

DG

5

DT

DA

DC

DA

DC

DT

DA

6

DC

DA

DC

DT

DC

DG

DC

DT

DA

7

DT

DG

DT

DA

DC

DA

8

DC

Entity 2, repressor 7 residues - Formula weight is not available

X=YOF

1

MET

YOF

HIS

TYR

LEU

Samples:

sample_1: operator0 – 0.0000375 mM; repressor 0.00003 mM; Tris 0.25 M; potassium chloride 0.2 M; EDTA 1 mM; dithiothreitol 1 mM; H2O 75%; D2O 25%

sample_2: operator0 – 0.0000375 mM; repressor 0.00003 mM; Tris 0.25 M; potassium chloride 0.5 M; EDTA 1 mM; dithiothreitol 1 mM; H2O 75%; D2O 25%

sample_3: operator0 – 0.0000375 mM; repressor 0.00003 mM; Tris 0.25 M; potassium chloride 0.5 M; EDTA 1 mM; dithiothreitol 1 mM; iPrSGal 0.0012 mM; H2O 75%; D2O 25%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
19F NMR	sample_1	isotropic	sample_conditions_1
19F NMR	sample_2	isotropic	sample_conditions_1
19F NMR	sample_3	isotropic	sample_conditions_1

Software:

software, na - na

NMR spectrometers:

Nicolet NT 141.2 MHz