BMRB Entry 17221

Name: Basic Requirements for a Metal-Binding Site in a Protein: The Influence of Loop Shortening on the Cupredoxin Azurin
Published: 2010-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17221

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Basic Requirements for a Metal-Binding Site in a Protein: The Influence of Loop Shortening on the Cupredoxin Azurin

Deposition date:

2010-09-29

Original release date:

2010-11-10

Authors:

Li, Chan; Yanagisawa, Sachiko; Martins, Berta; Messerschmidt, Albrecht; Banfield, Mark; Dennison, Christopher

Citation:

Citation: Li, Chan; Yanagisawa, Sachiko; Martins, Berta; Messerschmidt, Albrecht; Banfield, Mark. "Basic Requirements for a metal-binding site in a protein: The influence of loop shortening on the cupredoxin azurin" Proc. Natl. Acad. Sci. U.S.A. 103, 7258-7263 (2006).
PubMed: 16651527

Assembly members:

Assembly members:
AZAMIF, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: ns

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AZAMIF: AECSVDIQGNDQMQFNTNAI TVDKSCKQFTVNLSHPGNLP KNVMGHNWVLSTAADMQGVV TDGMASGLDKDYLKPDDSRV IAHTKLIGSGEKDSVTFDVS KLKEGEQYMFFCTPHPMKGT LTLK

Data sets:

chemical_rates
- Chemical_rate_list

Data type	Count
kinetic rates	2

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AZAMIF	1

Entities:

Entity 1, AZAMIF 124 residues - Formula weight is not available

1

ALA

GLU

CYS

SER

VAL

ASP

ILE

GLN

GLY

ASN

2

ASP

GLN

MET

GLN

PHE

ASN

THR

ASN

ALA

ILE

3

THR

VAL

ASP

LYS

SER

CYS

LYS

GLN

PHE

THR

4

VAL

ASN

LEU

SER

HIS

PRO

GLY

ASN

LEU

PRO

5

LYS

ASN

VAL

MET

GLY

HIS

ASN

TRP

VAL

LEU

6

SER

THR

ALA

ASP

MET

GLN

GLY

VAL

7

THR

ASP

GLY

MET

ALA

SER

GLY

LEU

ASP

LYS

8

ASP

TYR

LEU

LYS

PRO

ASP

SER

ARG

VAL

9

ILE

ALA

HIS

THR

LYS

LEU

ILE

GLY

SER

GLY

10

GLU

LYS

ASP

SER

VAL

THR

PHE

ASP

VAL

SER

11

LYS

LEU

LYS

GLU

GLY

GLU

GLN

TYR

MET

PHE

12

PHE

CYS

THR

PRO

HIS

PRO

MET

LYS

GLY

THR

13

LEU

THR

LEU

LYS

Samples:

sample_1: phosphate10 – 100 mM; AZAMIF 1:1 ratio; D2O 99.9%

sample_conditions_1: pH: 8.0; pressure: ambient atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H NMR	sample_1	isotropic	sample_conditions_1

Software:

PyMol, D eLano Scientific, San Carlos, CA - visualization

NMR spectrometers:

JEOL lambda 500 MHz