BMRB Entry 17219

Name: Structural Insights into the Monosaccharide Specificity of Escherichia coli Rhamnose Mutarotase
Published: 2010-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17219

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural Insights into the Monosaccharide Specificity of Escherichia coli Rhamnose Mutarotase

Deposition date:

2010-09-29

Original release date:

2010-11-10

Authors:

Ryu, Kyoung-Seok; Kim, Jung-In; Cho, Sung-Jae; Park, Dongkyu; Park, Chankyu; Cheong, Hae-Kap; Lee, Jie-Oh; Choi, Byong-Seok

Citation:

Citation: Ryu, Kyoung-Seok; Kim, Jung-In; Cho, Sung-Jae; Park, Dongkyu; Park, Chankyu; Cheong, Hae-Kap; Lee, Jie-Oh; Choi, Byong-Seok. "Structural Insights into the Monosaccharide Specificity of Escherichia coli Rhamnose Mutarotase" J. Mol. Biol. 349, 153-162 (2005).
PubMed: 15876375

Assembly members:

Assembly members:
YiiL, polymer, 104 residues, 164.156 Da.
RNS, non-polymer, 164.156 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YiiL: MIRKAFVMQVNPDAHEEYQR RHNPIWPELEAVLKSHGAHN YAIYLDKARNLLFAMVEIES EERWNAVASTDVCQRWWKYM TDVMPANPDNSPVSSELQEV FYLP

Data sets:

binding constants

Data type	Count
binding constants	2

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	YiiL_1	1
2	YiiL_2	1
3	RNS	2

Entities:

Entity 1, YiiL_1 104 residues - 164.156 Da.

1

MET

ILE

ARG

LYS

ALA

PHE

VAL

MET

GLN

VAL

2

ASN

PRO

ASP

ALA

HIS

GLU

TYR

GLN

ARG

3

ARG

HIS

ASN

PRO

ILE

TRP

PRO

GLU

LEU

GLU

4

ALA

VAL

LEU

LYS

SER

HIS

GLY

ALA

HIS

ASN

5

TYR

ALA

ILE

TYR

LEU

ASP

LYS

ALA

ARG

ASN

6

LEU

PHE

ALA

MET

VAL

GLU

ILE

GLU

SER

7

GLU

ARG

TRP

ASN

ALA

VAL

ALA

SER

THR

8

ASP

VAL

CYS

GLN

ARG

TRP

LYS

TYR

MET

9

THR

ASP

VAL

MET

PRO

ALA

ASN

PRO

ASP

ASN

10

SER

PRO

VAL

SER

GLU

LEU

GLN

GLU

VAL

11

PHE

TYR

LEU

PRO

Entity 2, RNS - C6 H12 O5 - 164.156 Da.

1

RNS

Samples:

sample_1: YiiL, [U-15N], 1 mM; RNS0 – 100 mM; D2O 100%

sample_conditions_1: pH: 7.5; pressure: ambient atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

software, na - na

NMR spectrometers:

Varian Unity Inova 600 MHz

PDB	1X8D
DBJ	BAB38251 BAE77408 BAG79713 BAI27848 BAI33322
EMBL	CAQ34253 CAR00877 CAR05531 CAR10574 CAR15556
GB	AAB03034 AAC76883 AAG59095 AAN45412 AAN83278
REF	NP_312855 NP_418337 NP_709705 WP_000619469 WP_000619484
SP	A1AI78 A7ZUB2 A8A704 B1IVH7 B1LMU1
AlphaFold	A1AI78 A7ZUB2 A8A704 B1IVH7 B1LMU1