BMRB Entry 17218

Title:
Enzyme Dynamics During Catalysis
Deposition date:
2010-09-29
Original release date:
2010-11-10
Authors:
Eisenmesser, Elan; Bosco, Daryl; Akke, Mikael; Kern, Dorothee
Citation:

Citation: Eisenmesser, Elan; Bosco, Daryl; Akke, Mikael; Kern, Dorothee. "Enzyme Dynamics During Catalysis"  Science 295, 1520-1523 (2002).
PubMed: 11859194

Assembly members:

Assembly members:
CypA, polymer, 164 residues, Formula weight is not available
peptide, polymer, 6 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ns

Data sets:
  • chemical rates
Data typeCount
binding constants8
kinetic rates7

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CypA1
2peptide2

Entities:

Entity 1, CypA 164 residues - Formula weight is not available

1   VALASNPROTHRVALPHEPHEASPILEALA
2   VALASPGLYGLUPROLEUGLYARGVALSER
3   PHEGLULEUPHEALAASPLYSVALPROLYS
4   THRALAGLUASNPHEARGALALEUSERTHR
5   GLYGLULYSGLYPHEGLYTYRLYSGLYSER
6   CYSPHEHISARGILEILEPROGLYPHEMET
7   CYSGLNGLYGLYASPPHETHRARGHISASN
8   GLYTHRGLYGLYLYSSERILETYRGLYGLU
9   LYSPHEGLUASPGLUASNPHEILELEULYS
10   HISTHRGLYPROGLYILELEUSERMETALA
11   ASNALAGLYPROASNTHRASNGLYSERGLN
12   PHEPHEILECYSTHRALALYSTHRGLUTRP
13   LEUASPGLYLYSHISVALVALPHEGLYLYS
14   VALLYSGLUGLYMETASNILEVALGLUALA
15   METGLUARGPHEGLYSERARGASNGLYLYS
16   THRSERLYSLYSILETHRILEALAASPCYS
17   GLYGLNLEUGLU

Entity 2, peptide 6 residues - Formula weight is not available

the first X is SIN and the other is NIT

1   SINALAPHEPROPHENIT

Samples:

sample_1: CypA 0.43 mM; sodium phosphate buffer 50 mM; DTT 3 mM; peptide0.04 – 2.6 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: ambient atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

Mathematica v4.0, Wolfram Research, Champaign, IL - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 2208 25104 25337 25502
PDB
DBJ BAE01146 BAE30323 BAE87660 BAF82774 BAF83540
EMBL CAA34961 CAA37039 CAA68264 CAG32988 CAH91833
GB AAB81959 AAB81960 AAB81961 AAF69142 AAF78600
PIR CSHYAC
PRF 1503232A
REF NP_001009370 NP_001027981 NP_001126060 NP_001270275 NP_001271703
SP P14851 P62935 P62936 P62937 P62938
TPG DAA25853 DAA25854
AlphaFold P62937 P62935 P62936 P14851 P62938