BMRB Entry 17211

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17211

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone and partial sidechain assignments of a fragment comprising transmembrane helix 1 to transmembrane helix 3 of the Ste2p receptor in TFE/water
Deposition date:
2010-09-27
Original release date:
2011-06-23
Authors:
Caroccia, Katrina; Arshava, Boris; Zerbe, Oliver; Naider, Fred
Citation:

Citation: Fracchiolla, Katrina; Poms, Martin; Arshava, Boris; Cohen, Leah; Zerbe, Oliver; Becker, Jeffrey; Naider, Fred. "Structural Characterization of Triple Transmembrane Domain Containing Fragments of a Yeast G Protein-Coupled Receptor in an Organic:Aqueous Environment by Solution-State NMR Spectroscopy"  Biopolymers ., .-..

Assembly members:

Assembly members:
Ste2p_TM1-TM3_(G31-R161), polymer, 131 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKC01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ste2p_TM1-TM3_(G31-R161): GNGSTITFDELQGLVNSTVT QAILFGVRSGAAALTLIVVW ITSRSRKTPIFIINQVSLFL IILHSALYFKYLLSNYSSVT YALTGFPQFISRGDVHVYGA TNIIQVLLVASIETSLVFQI KVIFTGDNFKR

Data sets:
Data typeCount
1H chemical shifts830
13C chemical shifts542
15N chemical shifts128

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fragment of Ste2p Receptor1

Entities:

Entity 1, Fragment of Ste2p Receptor 131 residues - Formula weight is not available

1   GLYASNGLYSERTHRILETHRPHEASPGLU
2   LEUGLNGLYLEUVALASNSERTHRVALTHR
3   GLNALAILELEUPHEGLYVALARGSERGLY
4   ALAALAALALEUTHRLEUILEVALVALTRP
5   ILETHRSERARGSERARGLYSTHRPROILE
6   PHEILEILEASNGLNVALSERLEUPHELEU
7   ILEILELEUHISSERALALEUTYRPHELYS
8   TYRLEULEUSERASNTYRSERSERVALTHR
9   TYRALALEUTHRGLYPHEPROGLNPHEILE
10   SERARGGLYASPVALHISVALTYRGLYALA
11   THRASNILEILEGLNVALLEULEUVALALA
12   SERILEGLUTHRSERLEUVALPHEGLNILE
13   LYSVALILEPHETHRGLYASPASNPHELYS
14   ARG

Samples:

sample_1: Ste2p TM1-TM3 (G31-R161), [U-95% 15N], 0.2 mM; trifluoroethanol 50%; H2O 50%

sample_2: Ste2p TM1-TM3 (G31-R161), [U-95% 13C; U-95% 15N; U-60% 2H], 0.29 mM; trifluoroethanol 50%; H2O 50%

sample_3: Ste2p TM1-TM3 (G31-R161) 0.42 mM; trifluoroethanol 50%; H2O 50%

sample_conditions_1: pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1

Software:

NMRView v5, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Direct Drive NMR System 600 MHz

Related Database Links:

BMRB 15995 17593
PDB
DBJ BAA09212 GAA23058
EMBL CAA26794 CAD56352 CAD56353 CAD56354 CAD56355
GB AAA35112 AAT93153 AJU36009 AJU36114 AJU36830
PRF 1112154A
REF NP_116627
SP D6VTK4 P0CI39
TPG DAA12414
AlphaFold D6VTK4 P0CI39

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks