BMRB Entry 17205

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17205

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Title:
Chemical shift of fully reduced Cox17 from Yeast.
Deposition date:
2010-09-24
Original release date:
2011-03-07
Authors:
Bertini, Ivano; Felli, Isabella C; Gonelli, Leonardo; Machohally Venkateshaiah, Vasantha Kumar; Pierttelli, Roberta
Citation:

Citation: Bertini, Ivano; Felli, Isabella; Gonnelli, Leonardo; Kumar M, Vasantha; Pierattelli, Roberta. "(13) C direct-detection biomolecular NMR spectroscopy in living cells."  Angew. Chem. Int. Ed. Engl. 50, 2339-2341 (2011).
PubMed: 21351349

Assembly members:

Assembly members:
Cytochrom_C_oxidase_chaperone, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cytochrom_C_oxidase_chaperone: MTETDKKQEQENHAECEDKP KPCCVCKPEKEERDTCILFN GQDSEKCKEFIEKYKECMKG YGFEVPSAN

Data sets:
Data typeCount
13C chemical shifts201
15N chemical shifts67
1H chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cox171

Entities:

Entity 1, Cox17 69 residues - Formula weight is not available

1   METTHRGLUTHRASPLYSLYSGLNGLUGLN
2   GLUASNHISALAGLUCYSGLUASPLYSPRO
3   LYSPROCYSCYSVALCYSLYSPROGLULYS
4   GLUGLUARGASPTHRCYSILELEUPHEASN
5   GLYGLNASPSERGLULYSCYSLYSGLUPHE
6   ILEGLULYSTYRLYSGLUCYSMETLYSGLY
7   TYRGLYPHEGLUVALPROSERALAASN

Samples:

Cox17y: Cytochrom C oxidase chaperone, [U-13C; U-15N], 1.8 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D (H)CBCACON-IPAPCox17yisotropicsample_conditions_1
3D (H)NCANCO-IPAPCox17yisotropicsample_conditions_1
3D (H)CBCANCO-IPAPCox17yisotropicsample_conditions_1
3D COCON-IPAPCox17yisotropicsample_conditions_1
3D HNCOCox17yisotropicsample_conditions_1
3D HNCANNHCox17yisotropicsample_conditions_1
3D CBCA(CO)NHCox17yisotropicsample_conditions_1
3D CBCANHCox17yisotropicsample_conditions_1
3D CC(CO)NHCox17yisotropicsample_conditions_1
2D 1H-15N HSQCCox17yisotropicsample_conditions_1
2D CACOCox17yisotropicsample_conditions_1
2D CONCox17yisotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin, Keller and Wuthrich - collection, data analysis, processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance 700 MHz

Related Database Links:

UNP Q12287
AlphaFold D6VXZ3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks