BMRB Entry 17195

Name: Backbone assignments for the UBA Domain of E2-25K
Published: 2011-03-24

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17195

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments for the UBA Domain of E2-25K

Deposition date:

2010-09-15

Original release date:

2011-03-24

Authors:

Wilson, Randall; Edmondson, Stephen; Flatt, Justin; Twigg, Pamela

Citation:

Citation: Wilson, Randall; Edmondson, Stephen; Flatt, Justin; Helms, Kimberli; Twigg, Pamela. "The E2-25K ubiquitin-associated (UBA) domain aids in polyubiquitin chain synthesis and linkage specificity" Biochem. Biophys. Res. Commun. 405, 662-666 (2011).
PubMed: 21281599

Assembly members:

Assembly members:
E2-25K_UBA_domain, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E2-25K_UBA_domain: MGSSHHHHHHSSGLVPEGSH MASGAPVSSPEYTKKIENLC AMGFDRNAVIVALSSKSWDV ETATELLLSN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
RDCs
- RDC_list_1

Data type	Count
13C chemical shifts	155
15N chemical shifts	50
1H chemical shifts	50
residual dipolar couplings	37

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBA monomer	1

Entities:

Entity 1, UBA monomer 70 residues - Formula weight is not available

Residues 1-23 represent a non-native affinity tag and residues 24-70 represent residues 154-200 of E2-25K

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	GLU	GLY	SER	HIS
3	MET	ALA	SER	GLY	ALA	PRO	VAL	SER	SER	PRO
4	GLU	TYR	THR	LYS	LYS	ILE	GLU	ASN	LEU	CYS
5	ALA	MET	GLY	PHE	ASP	ARG	ASN	ALA	VAL	ILE
6	VAL	ALA	LEU	SER	SER	LYS	SER	TRP	ASP	VAL
7	GLU	THR	ALA	THR	GLU	LEU	LEU	LEU	SER	ASN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D Nhsqc-ipap	RDC_sample	isotropic	RDC_conditions
2D NHSC S3	RDC_sample	isotropic	RDC_conditions

BMRB Entry 17195

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	GLU	GLY	SER	HIS
3	MET	ALA	SER	GLY	ALA	PRO	VAL	SER	SER	PRO
4	GLU	TYR	THR	LYS	LYS	ILE	GLU	ASN	LEU	CYS
5	ALA	MET	GLY	PHE	ASP	ARG	ASN	ALA	VAL	ILE
6	VAL	ALA	LEU	SER	SER	LYS	SER	TRP	ASP	VAL
7	GLU	THR	ALA	THR	GLU	LEU	LEU	LEU	SER	ASN

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	GLU	GLY	SER	HIS
3	MET	ALA	SER	GLY	ALA	PRO	VAL	SER	SER	PRO
4	GLU	TYR	THR	LYS	LYS	ILE	GLU	ASN	LEU	CYS
5	ALA	MET	GLY	PHE	ASP	ARG	ASN	ALA	VAL	ILE
6	VAL	ALA	LEU	SER	SER	LYS	SER	TRP	ASP	VAL
7	GLU	THR	ALA	THR	GLU	LEU	LEU	LEU	SER	ASN

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	GLU	GLY	SER	HIS
3	MET	ALA	SER	GLY	ALA	PRO	VAL	SER	SER	PRO
4	GLU	TYR	THR	LYS	LYS	ILE	GLU	ASN	LEU	CYS
5	ALA	MET	GLY	PHE	ASP	ARG	ASN	ALA	VAL	ILE
6	VAL	ALA	LEU	SER	SER	LYS	SER	TRP	ASP	VAL
7	GLU	THR	ALA	THR	GLU	LEU	LEU	LEU	SER	ASN