BMRB Entry 17194

Title:
Oxki4a, spider derived antimicrobial peptide
Deposition date:
2010-09-14
Original release date:
2012-08-01
Authors:
Vassilevski, Alexander; Dubovskii, Peter; Samsonova, Olga; Egorova, Natalya; Kozlov, Sergey; Feofanov, Alexei; Arseniev, Alexander; Grishin, Eugene
Citation:

Citation: Dubovskii, Peter; Vassilevski, Alexander; Samsonova, Olga; Egorova, Natalya; Kozlov, Sergey; Feofanov, Alexei; Arseniev, Alexander; Grishin, Eugene. "Novel lynx spider toxin shares common molecular architecture with defense peptides from frog skin"  FEBS J. 278, 4382-4393 (2011).
PubMed: 21933345

Assembly members:

Assembly members:
entity, polymer, 30 residues, 3624.556 Da.

Natural source:

Natural source:   Common Name: lynx spider   Taxonomy ID: 666126   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oxyopes takobius

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GIRCPKSWKCKAFKQRVLKR LLAMLRQHAF

Data sets:
Data typeCount
1H chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Oxki4a1

Entities:

Entity 1, Oxki4a 30 residues - 3624.556 Da.

1   GLYILEARGCYSPROLYSSERTRPLYSCYS
2   LYSALAPHELYSGLNARGVALLEULYSARG
3   LEULEUALAMETLEUARGGLNHISALAPHE

Samples:

sample_1: Oxki4a 1 ± 0.1 mM; dodecylphosphocholine-d38 60 ± 1 mM; D2O 5%; H2O 95%

sample-2: Oxki4a 1 mM; dodecylphosphocholine-d38 60 mM; D2O 100%

sample_conditions_1: ionic strength: 0 M; pH: 4.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample-2isotropicsample_conditions_1
2D 1H-1H NOESYsample-2isotropicsample_conditions_1
2D DQF-COSYsample-2isotropicsample_conditions_1

Software:

XEASY, Bartels C., Xia T., Billeter M., Guntert P., Wuthrich K. - chemical shift assignment

xwinnmr, Bruker Biospin - collection

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
EMBL CBQ82558
SP F8J4S0
AlphaFold F8J4S0