BMRB Entry 17192

Name: Solution NMR Structure of CH domain of Rho guanine nucleotide exchange factor 7 from Homo sapiens, Northeast Structural Genomics Consortium Target HR4495E
Published: 2010-10-13

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17192

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR Structure of CH domain of Rho guanine nucleotide exchange factor 7 from Homo sapiens, Northeast Structural Genomics Consortium Target HR4495E

Deposition date:

2010-09-13

Original release date:

2010-10-13

Authors:

Liu, Gaohua; Xiao, Rong; Janjua, Haleema; Acton, Thomas; Ciccosanti, Colleen; Shastry, Ritu; Everett, John; Montelione, Gaetano

Citation:

Citation: Liu, Gaohua; Xiao, Rong; Janjua, Haleema; Ciccosanti, Colleen; Shastry, Ritu; Acton, Thomas; Everett, John; Montelione, Gaetano. "Northeast Structural Genomics Consortium Target HR4495E" To be published ., .-..

Assembly members:

Assembly members:
HR4495E, polymer, 126 residues, 14070.131 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 14-15C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR4495E: MGHHHHHHSHMNSAEQTVTW LITLGVLESPKKTISDPEGF LQASLKDGVVLCRLLERLLP GTIEKVYPEPRSESECLSNI REFLRGCGASLRLETFDAND LYQGQNFNKVLSSLVTLNKV TADIGL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	518
15N chemical shifts	128
1H chemical shifts	856

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HR4495E	1

Entities:

Entity 1, HR4495E 126 residues - 14070.131 Da.

sequence contains N-terminal His-tag: MGHHHHHHSHM

1

MET

GLY

HIS

SER

HIS

2

MET

ASN

SER

ALA

GLU

GLN

THR

VAL

THR

TRP

3

LEU

ILE

THR

LEU

GLY

VAL

LEU

GLU

SER

PRO

4

LYS

THR

ILE

SER

ASP

PRO

GLU

GLY

PHE

5

LEU

GLN

ALA

SER

LEU

LYS

ASP

GLY

VAL

6

LEU

CYS

ARG

LEU

GLU

ARG

LEU

PRO

7

GLY

THR

ILE

GLU

LYS

VAL

TYR

PRO

GLU

PRO

8

ARG

SER

GLU

SER

GLU

CYS

LEU

SER

ASN

ILE

9

ARG

GLU

PHE

LEU

ARG

GLY

CYS

GLY

ALA

SER

10

LEU

ARG

LEU

GLU

THR

PHE

ASP

ALA

ASN

ASP

11

LEU

TYR

GLN

GLY

GLN

ASN

PHE

ASN

LYS

VAL

12

LEU

SER

LEU

VAL

THR

LEU

ASN

LYS

VAL

13

THR

ALA

ASP

ILE

GLY

LEU

Samples:

sample_NC: HR4495E, [U-100% 13C; U-100% 15N], 0.87 mM; H2O 95%; D2O 5%

sample_NC5: HR4495E, [U-5% 13C; U-100% 15N], 0.85 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_NC	isotropic	sample_conditions_1
2D 1H-13C HSQC ct 28	sample_NC5	isotropic	sample_conditions_1
3D HNCO	sample_NC	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_NC	isotropic	sample_conditions_1
3D HNCACB	sample_NC	isotropic	sample_conditions_1
3D 1H-13C arom NOESY	sample_NC	isotropic	sample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY	sample_NC	isotropic	sample_conditions_1
2D 1H-13C HSQC ct 42	sample_NC5	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_NC	isotropic	sample_conditions_1
3D CC(CO)NH TOCSY	sample_NC	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_NC	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinemen, structure solution

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinemen, structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis, refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

NMR spectrometers:

Bruker Avance 800 MHz
Varian INOVA 600 MHz

PDB	2L3G
DBJ	BAC97874 BAE23905
EMBL	CAD38906
GB	ACE87830 ACT64348 EAX09145 EAX09148 ELK14885
REF	NP_001106989 NP_663788 XP_001365011 XP_003778289 XP_004054793
SP	Q9ES28
AlphaFold	Q9ES28