BMRB Entry 17187
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17187
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Citation: Archer, Sharon; Vinson, Valda; Pollard, Thomas; Torchia, Dennis. "Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy" FEBS Lett. 337, 145-151 (1994).
PubMed: 8287969
Assembly members:
profilinI, polymer, 126 residues, Formula weight is not available
pLp, polymer, 10 residues, Formula weight is not available
Natural source: Common Name: Acanthamoeba castellanii Taxonomy ID: 5755 Superkingdom: Eukaryota Kingdom: not available Genus/species: Acanthamoeba castellanii
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
profilinI: MSWQTYVDTNLVGTGAVTQA
AILGLDGNTWATSAGFAVTP
AQGQTLASAFNNADPIRASG
FDLAGVHYVTLRADDRSIYG
KKGSAGVITVKTSKSILVGV
YNEKIQPGTAANVVEKLADY
LIGQGF
pLp: PPPPPPPPPP
- binding constants
| Data type | Count |
| binding constants | 8 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | profilinI | 1 |
| 2 | pLp | 2 |
Entities:
Entity 1, profilinI 126 residues - Formula weight is not available
| 1 | MET | SER | TRP | GLN | THR | TYR | VAL | ASP | THR | ASN | ||||
| 2 | LEU | VAL | GLY | THR | GLY | ALA | VAL | THR | GLN | ALA | ||||
| 3 | ALA | ILE | LEU | GLY | LEU | ASP | GLY | ASN | THR | TRP | ||||
| 4 | ALA | THR | SER | ALA | GLY | PHE | ALA | VAL | THR | PRO | ||||
| 5 | ALA | GLN | GLY | GLN | THR | LEU | ALA | SER | ALA | PHE | ||||
| 6 | ASN | ASN | ALA | ASP | PRO | ILE | ARG | ALA | SER | GLY | ||||
| 7 | PHE | ASP | LEU | ALA | GLY | VAL | HIS | TYR | VAL | THR | ||||
| 8 | LEU | ARG | ALA | ASP | ASP | ARG | SER | ILE | TYR | GLY | ||||
| 9 | LYS | LYS | GLY | SER | ALA | GLY | VAL | ILE | THR | VAL | ||||
| 10 | LYS | THR | SER | LYS | SER | ILE | LEU | VAL | GLY | VAL | ||||
| 11 | TYR | ASN | GLU | LYS | ILE | GLN | PRO | GLY | THR | ALA | ||||
| 12 | ALA | ASN | VAL | VAL | GLU | LYS | LEU | ALA | ASP | TYR | ||||
| 13 | LEU | ILE | GLY | GLN | GLY | PHE |
Entity 2, pLp 10 residues - Formula weight is not available
| 1 | PRO | PRO | PRO | PRO | PRO | PRO | PRO | PRO | PRO | PRO |
Samples:
sample_1: profilinI, [U-13C; U-15N], 0.1 mM; pLp0 – 8 mM; H2O 93%; D2O 7%
sample_2: profilinI, [U-13C; U-15N], 1.0 mM; pLp 0-8 mM; H2O 93%; D2O 7%
sample_conditions_1: pH: 6.4; pressure: ambient atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
inhouse, F. Delaglio, nmrpipe , unpublished) - processing
NMRi, (New Methods Research Inc., Syracuse, NY - processing
inhouse2, Garrett, D.S., Powers, R., Gronenborn, A.M. and Clore, G.M. - data analysis
NMR spectrometers:
- Bruker AMX 500 MHz