BMRB Entry 17182

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17182

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Title:
Weak Substrate Binding to Transport Proteins Studied by NMR
Deposition date:
2010-09-10
Original release date:
2010-11-10
Authors:
Spooner, Paul; O'Reilly, W.; Homans, Steven; Rutherford, Nicholas; Henderson, Peter; Watts, Anthony
Citation:

Citation: Spooner, Paul; O'Reilly, W.; Homans, Steven; Rutherford, Nicholas; Henderson, Peter; Watts, Anthony. "Weak Substrate Binding to Transport Proteins Studied by NMR"  Biophys. J. 75, 2794-2800 (1998).
PubMed: 9826601

Assembly members:

Assembly members:
FucP, polymer, 438 residues, 47545 Da.
ARA, D-SACCHARIDE, 150.130 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FucP: MGNTSIQTQSYRAVDKDAGQ SRSYIIPFALLCSLFFLWAV ANNLNDILLPQFQQAFTLTN FQAGLIQSAFYFGYFIIPIP AGILMKKLSYKAGIITGLFL YALGAALFWPAAEIMNYTLF LVGLFIIAAGLGCLETAANP FVTVLGPESSGHFRLNLAQT FNSFGAIIAVVFGQSLILSN VPHQSQDVLDKMSPEQLSAY KHSLVLSVQTPYMIIVAIVL LVALLIMLTKFPALQSDNHS DAKQGSFSASLSRLARIRHW RWAVLAQFCYVGAQTACWSY LIRYAVEEIPGMTAGFAANY LTGTMVCFFIGRFTGTWLIS RFAPHKVLAAYALIAMALCL ISAFAGGHVGLIALTLCSAF MSIQYPTIFSLGIKNLGQDT KYGSSFIVMTIIGGGIVTPV MGFVSDAAGNIPTAELIPAL CFAVIFIFARFRSQTATN

Data sets:
Data typeCount
kinetic rates1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FucP1
2arabinose2

Entities:

Entity 1, FucP 438 residues - 47545 Da.

1   METGLYASNTHRSERILEGLNTHRGLNSER
2   TYRARGALAVALASPLYSASPALAGLYGLN
3   SERARGSERTYRILEILEPROPHEALALEU
4   LEUCYSSERLEUPHEPHELEUTRPALAVAL
5   ALAASNASNLEUASNASPILELEULEUPRO
6   GLNPHEGLNGLNALAPHETHRLEUTHRASN
7   PHEGLNALAGLYLEUILEGLNSERALAPHE
8   TYRPHEGLYTYRPHEILEILEPROILEPRO
9   ALAGLYILELEUMETLYSLYSLEUSERTYR
10   LYSALAGLYILEILETHRGLYLEUPHELEU
11   TYRALALEUGLYALAALALEUPHETRPPRO
12   ALAALAGLUILEMETASNTYRTHRLEUPHE
13   LEUVALGLYLEUPHEILEILEALAALAGLY
14   LEUGLYCYSLEUGLUTHRALAALAASNPRO
15   PHEVALTHRVALLEUGLYPROGLUSERSER
16   GLYHISPHEARGLEUASNLEUALAGLNTHR
17   PHEASNSERPHEGLYALAILEILEALAVAL
18   VALPHEGLYGLNSERLEUILELEUSERASN
19   VALPROHISGLNSERGLNASPVALLEUASP
20   LYSMETSERPROGLUGLNLEUSERALATYR
21   LYSHISSERLEUVALLEUSERVALGLNTHR
22   PROTYRMETILEILEVALALAILEVALLEU
23   LEUVALALALEULEUILEMETLEUTHRLYS
24   PHEPROALALEUGLNSERASPASNHISSER
25   ASPALALYSGLNGLYSERPHESERALASER
26   LEUSERARGLEUALAARGILEARGHISTRP
27   ARGTRPALAVALLEUALAGLNPHECYSTYR
28   VALGLYALAGLNTHRALACYSTRPSERTYR
29   LEUILEARGTYRALAVALGLUGLUILEPRO
30   GLYMETTHRALAGLYPHEALAALAASNTYR
31   LEUTHRGLYTHRMETVALCYSPHEPHEILE
32   GLYARGPHETHRGLYTHRTRPLEUILESER
33   ARGPHEALAPROHISLYSVALLEUALAALA
34   TYRALALEUILEALAMETALALEUCYSLEU
35   ILESERALAPHEALAGLYGLYHISVALGLY
36   LEUILEALALEUTHRLEUCYSSERALAPHE
37   METSERILEGLNTYRPROTHRILEPHESER
38   LEUGLYILELYSASNLEUGLYGLNASPTHR
39   LYSTYRGLYSERSERPHEILEVALMETTHR
40   ILEILEGLYGLYGLYILEVALTHRPROVAL
41   METGLYPHEVALSERASPALAALAGLYASN
42   ILEPROTHRALAGLULEUILEPROALALEU
43   CYSPHEALAVALILEPHEILEPHEALAARG
44   PHEARGSERGLNTHRALATHRASN

Entity 2, arabinose - 150.130 Da.

1   ARA

Samples:

sample_1: arabinose, [U-13C], 150.13 ug; FucP 9.5 mg; D-fucose164.2 – 492.5 ug

sample_conditions_1: pH: 7.5; pressure: ambient atm; temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
13C CP MASsample_1anisotropicsample_conditions_1

Software:

software, na - na

NMR spectrometers:

  • Bruker MSL 400 MHz

Related Database Links:

PDB
DBJ BAB37084 BAE76873 BAG78585 BAI27062 BAI32091
EMBL CAA33126 CAP77234 CAQ99729 CAR04311 CAR09414
GB AAB40451 AAC75843 AAG57915 AAN44303 AAN81817
REF NP_311688 NP_417281 NP_708596 WP_000528574 WP_000528575
SP P11551
AlphaFold P11551